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PMID: 6186992 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cross-reaction of snRNA and an Alu I-like sequence from rat with DNAs from different eucaryotic species.

Nucleic acids research ·Vol. 11 ·No. 5 ·1983-03-11 ·Pages 1375-88

Blin N, Weber T, Alonso A

Abstract

Sequence homologies to rat U1-snRNA and U2-snRNA were investigated in DNA from 23 eucaryotic species (3 lower eucaryotes, 4 plants, and 16 animals) using dot hybridization at various stringency conditions. Cross-hybridization among very distantly related species in e.g. plants-insects or mold-vertebrates is not the rule; there are, however, examples for stronger homologies like Rattus-Dictyostelium. Furthermore, DNA from all 23 species was analysed for sequence homologies with the repetitive DNA sequence B1 (an Alu I family equivalent) from rat. We observed a wide range of homologies covering some plants and insects, up to vertebrates. Hybridization at increasing stringency conditions revealed species with higher degree of homology to the rat B1 sequence: maize, chicken, and hamster.

MeSH Terms
Animals Base Sequence DNA/genetics DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific Female Fungi/genetics Humans Nucleic Acid Denaturation Nucleic Acid Hybridization Organ Specificity Plants/genetics Pregnancy RNA/genetics RNA, Small Nuclear Rats Repetitive Sequences, Nucleic Acid Species Specificity
Chemicals
RNA, Small Nuclear RNA DNA DNA Restriction Enzymes endodeoxyribonuclease AluI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blin N
Weber T
Alonso A
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.5.1375
Published
1983-03-11
Pages
1375-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325803
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.5.1375 PMC Full Text View journal details
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