Home LiteratureArticle Details
PMID: 6190978 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Three T cell hybridomas do not contain detectable heavy chain variable gene transcripts.

The Journal of experimental medicine ·Vol. 158 ·No. 1 ·1983-07-01 ·Pages 210-27

Kronenberg M, Kraig E, Siu G, Kapp JA, Kappler J, Marrack P, Pierce CW, Hood L

Abstract

We attempted to determine whether T cells express any VH gene segments. cDNA libraries were constructed from one suppressor and two helper T cell hybridomas. Both the library construction and screening were designed to maximize detection of a wide range of VH gene segments. One screening method should detect about half of the sequenced VH genes, while the second should detect most of these genes. The probability of detecting a VH gene homologous to the probes and present at 10 copies per cell was 77% for one helper cell cDNA library, 88% for the second helper cell library, and greater than 99% for the suppressor cell library. No cDNA clones with VH gene segments were detected. From this result, we conclude that VH gene segments are not likely to encode the antigen-specific receptor in the cells we tested.

MeSH Terms
Animals Antigens/immunology Binding Sites, Antibody/genetics DNA DNA, Recombinant Hemocyanins Hybridomas/immunology Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Mice Mice, Inbred BALB C Nucleic Acid Hybridization Peptides/immunology Polymers Receptors, Antigen/genetics T-Lymphocytes/immunology Transcription, Genetic gamma-Globulins/immunology
Chemicals
Antigens DNA, Recombinant Immunoglobulin Heavy Chains Immunoglobulin Variable Region Peptides Polymers Receptors, Antigen gamma-Globulins GAT DNA Hemocyanins keyhole-limpet hemocyanin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kronenberg M
Kraig E
Siu G
Kapp J A
Kappler J
Marrack P
Pierce C W
Hood L
References (70)
70 references, click to expand
  1. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  2. 2 -Microglobulin--a free immunoglobulin domain.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1697-701 PMID: 4558655
  3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  4. The organization, expression, and evolution of antibody genes and other multigene families.
    Annu Rev Genet. 1975;9:305-53 PMID: 813561
  5. Idiotypic analysis of lymphocytes in vitro. II. Genetic control of T-helper cell responsiveness to anti-idiotypic antibody.
    J Exp Med. 1976 Apr 1;143(4):861-9 PMID: 56419
  6. Complexity and specificity of polysomal poly(A+) RNA in mouse tissues.
    Biochemistry. 1976 Jun 29;15(13):2823-9 PMID: 985697
  7. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase.
    Biochim Biophys Acta. 1976 Sep 6;442(3):324-30 PMID: 183818
  8. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  9. T-cell idiotypes are linked to immunoglobulin heavy chain genes.
    Nature. 1976 Dec 16;264(5587):639-42 PMID: 137363
  10. Two different VH gene products make up the T-cell receptors.
    Scand J Immunol. 1976;5(9):993-1001 PMID: 63988
  11. The expression of three abundance classes of messenger RNA in mouse tissues.
    Cell. 1976 Dec;9(4 PT 2):761-74 PMID: 1017013
  12. Antigen-binding, idiotypic T-lymphocyte receptors.
    Contemp Top Immunobiol. 1977;7:113-77 PMID: 69514
  13. Antigen receptors of T helper cells.
    Contemp Top Immunobiol. 1977;7:69-112 PMID: 69518
  14. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  15. Synthesis of full length cDNAs from four partially purified oviduct mRNAs.
    J Biol Chem. 1978 Apr 10;253(7):2471-82 PMID: 632280
  16. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
    J Biol Chem. 1978 Apr 10;253(7):2483-95 PMID: 75887
  17. Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1485-9 PMID: 418414
  18. A bacterial clone synthesizing proinsulin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3727-31 PMID: 358198
  19. Sequences of mouse immunoglobulin light chain genes before and after somatic changes.
    Cell. 1978 Dec;15(4):1133-44 PMID: 103630
  20. Mechanisms of regulation of cell-mediated immunity. IV. Azobenzenearsonate-specific suppressor factor(s) bear cross-reactive idiotypic determinants the expression of which is linked to the heavy-chain allotype linkage group of genes.
    J Exp Med. 1979 May 1;149(5):1084-98 PMID: 87484
  21. Methylmercury hydroxide enhancement of translation and transcription of ovalbumin and conalbumin mRNA's.
    J Biol Chem. 1979 Aug 25;254(16):7636-42 PMID: 89113
  22. The heavy chain of human histocompatibility antigen HLA-B7 contains an immunoglobulin-like region.
    Nature. 1979 Nov 15;282(5736):266-70 PMID: 388231
  23. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  24. Immunoglobulin messenger RNAs in murine cell lines that have characteristics of immature B lymphocytes.
    Cell. 1979 Dec;18(4):1333-9 PMID: 117905
  25. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  26. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  27. 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate.
    Gene. 1980 Jul;10(2):177-83 PMID: 6248422
  28. Expression of the immunoglobulin C mu gene in mouse T and B lymphoid and myeloid cell lines.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2876-80 PMID: 6771761
  29. A mature T lymphocyte subpopulation marker closely linked to the Ig-1 allotype Ch locus.
    Eur J Immunol. 1979 Dec;9(12):948-55 PMID: 317707
  30. The immunoglobulin mu constant region gene is expressed in mouse thymocytes.
    Nature. 1980 Jul 10;286(5769):168-70 PMID: 6772961
  31. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  32. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  33. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  34. An immunoglobulin heavy-chain gene is altered in two T-cell clones.
    Nature. 1980 Aug 28;286(5776):897-9 PMID: 6774263
  35. Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3630-4 PMID: 6251474
  36. Cloning and sequence of the cDNA corresponding to the variable region of immunoglobulin heavy chain MPC11.
    Nucleic Acids Res. 1980 Aug 25;8(16):3591-601 PMID: 6253904
  37. Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain.
    Nucleic Acids Res. 1980 Mar 25;8(6):1231-41 PMID: 6253932
  38. The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers.
    Nucleic Acids Res. 1980 May 10;8(9):1913-31 PMID: 6159597
  39. Somatic rearrangements forming active immunoglobulin mu genes in B and T lymphoid cell lines.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4943-7 PMID: 6776532
  40. Helper and killer T cells do not express B cell immunoglobulin joining and constant region gene segments.
    J Exp Med. 1980 Dec 1;152(6):1745-61 PMID: 6778952
  41. The isolation of cloned cDNA sequences which are differentially expressed in human lymphocytes and fibroblasts.
    Nucleic Acids Res. 1980 Dec 20;8(24):6007-17 PMID: 6162155
  42. Transcripts of the immunoglobulin C mu gene vary in structure and splicing during lymphoid development.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7400-4 PMID: 6784123
  43. Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes.
    Cell. 1981 Apr;24(1):125-34 PMID: 6786753
  44. Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.
    Cell. 1981 Jun;24(3):625-37 PMID: 6788376
  45. The T suppressor cell alloantigen Tsud maps near immunoglobulin allotype genes and may be an heavy chain constant-region marker on a T cell receptor.
    J Exp Med. 1981 Apr 1;153(4):801-10 PMID: 6454740
  46. Diversity of germ-line immunoglobulin VH genes.
    Nature. 1981 Jul 30;292(5822):426-30 PMID: 6789211
  47. Immunoglobulin genes undergo multiple sequence rearrangements during differentiation.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:867-78 PMID: 6790219
  48. A single VH gene segment encodes the immune response to phosphorylcholine: somatic mutation is correlated with the class of the antibody.
    Cell. 1981 Jul;25(1):59-66 PMID: 6791833
  49. A family of T-cell alloantigens linked to Igh-1.
    Nature. 1981 Oct 29;293(5835):742-5 PMID: 6974825
  50. Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.
    Nucleic Acids Res. 1981 Aug 25;9(16):4099-109 PMID: 6795591
  51. The T-cell receptor problem.
    Curr Top Microbiol Immunol. 1981;91:179-215 PMID: 6796337
  52. The nucleotide sequences of rearranged and germline immunoglobulin VH genes of a mouse myeloma MC101 and evolution of VH genes in mouse.
    J Biol Chem. 1982 Jan 10;257(1):277-85 PMID: 6273429
  53. Two distinct allotypic determinants on the antigen-specific suppressor and enhancing T cell factors that are encoded by genes linked to the immunoglobulin heavy chain locus.
    J Exp Med. 1982 Jan 1;155(1):126-39 PMID: 6172538
  54. Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain.
    Science. 1982 Apr 16;216(4543):309-11 PMID: 6801765
  55. The 5936 Ig-idiotype(s): genetic linkage to Ig-Ch Loci, T-cell dependence of synthesis and possible specificities.
    Scand J Immunol. 1981 Dec;14(6):673-85 PMID: 6805069
  56. Antigen-specific, H-2-restricted helper T cell hybridomas.
    J Exp Med. 1982 Jul 1;156(1):191-204 PMID: 6177819
  57. Reconstitution of antigen-specific suppressor activity with translation products of mRNA.
    Nature. 1982 Jul 8;298(5870):172-4 PMID: 6211624
  58. Constant region determinants on the antigen-binding chain of the suppressor T-cell factor.
    Nature. 1982 Jul 8;298(5870):174-6 PMID: 6178037
  59. Simultaneous expression of immunoglobulin mu and delta heavy chains by a cloned B-cell lymphoma: a single copy of the VH gene is shared by two adjacent CH genes.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2996-3000 PMID: 6806821
  60. Immunoglobulin heavy chain gene rearrangement and transcription in murine T cell hybrids and T lymphomas.
    Proc Natl Acad Sci U S A. 1982 May;79(9):3015-9 PMID: 6806823
  61. Simple DNA sequences in homologous flanking regions near immunoglobulin VH genes: a role in gene interaction?
    Nucleic Acids Res. 1982 Jun 11;10(11):3353-70 PMID: 6285297
  62. Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3687-91 PMID: 6954511
  63. Immunoglobulin idiotopes expressed by T cells. I. Expression of distinct idiotopes detected by monoclonal antibodies on antigen-specific suppressor T cells.
    J Exp Med. 1982 Sep 1;156(3):719-30 PMID: 6213731
  64. Organization and evolution of immunoglobulin VH gene subgroups.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4405-9 PMID: 6812048
  65. Alpha chain of HLA-DR transplantation antigens is a member of the same protein superfamily as the immunoglobulins.
    Cell. 1982 Aug;30(1):153-61 PMID: 6812963
  66. A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells.
    J Exp Med. 1982 Dec 1;156(6):1848-53 PMID: 7175441
  67. A T15-idiotype-positive T suppressor hybridoma does not use the T15 VH gene segment.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6984-8 PMID: 6983692
  68. T and B cells that recognize the same antigen do not transcribe similar heavy chain variable region gene segments.
    J Exp Med. 1983 Jul 1;158(1):192-209 PMID: 6190977
  69. Immunoglobulin gene rearrangements in normal mouse B cells.
    Mol Cell Biol. 1982 Jul;2(7):829-36 PMID: 6821506
  70. Antibody diversity: somatic hypermutation of rearranged VH genes.
    Cell. 1981 Dec;27(3 Pt 2):573-81 PMID: 6101208
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.158.1.210
Published
1983-07-01
Pages
210-27
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187070
Subset
IM
Grants
NIAID NIH HHS · AI 15353 · United States
NIAID NIH HHS · AI 17565 · United States
NIAID NIH HHS · AI 18959 · United States
External Links
PubMed source DOI: 10.1084/jem.158.1.210 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2