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PMID: 6253889 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alterations in two conserved regions of promoter sequence lead to altered rates of polymerase binding and levels of gene expression.

Nucleic acids research ·Vol. 8 ·No. 12 ·1980-06-25 ·Pages 2709-23

Stefano JE, Ackerson JW, Gralla JD

Abstract

Characterization of recombinant lac promoters highlights the importance of two regions of sequence conservation in promoters. The "Pribnow box" sequences are necessary for specific transcription in this system. This specificity is maintained when a mutated upstream sequence is introduced. However, changing the upstream DNA sequences influences both the rate of RNA polymerase binding in vitro and levels of expression in vivo.

MeSH Terms

Base Sequence DNA Restriction Enzymes DNA, Recombinant/metabolism DNA-Directed RNA Polymerases/metabolism Drug Resistance, Microbial Escherichia coli/drug effects,enzymology Kinetics Lac Operon Mutation Protein Binding Protein Biosynthesis Tetracycline/pharmacology Transcription, Genetic

Chemicals

DNA, Recombinant DNA-Directed RNA Polymerases DNA Restriction Enzymes Tetracycline

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Stefano J E

Ackerson J W

Gralla J D

References (27)

27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.12.2709

Published

1980-06-25

Pages

2709-23

Language

English

Region

England

NLM ID

0411011

PMCID

PMC324115

Subset

Grants

NCI NIH HHS · CA 19941 · United States

External Links

PubMed source DOI: 10.1093/nar/8.12.2709 PMC Full Text View journal details

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Alterations in two conserved regions of promoter sequence lead to altered rates of polymerase binding and levels of gene expression.

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