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PMID: 6263867 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Absence in Bacillus subtilis and Staphylococcus aureus of the sequence-specific deoxyribonucleic acid methylation that is conferred in Escherichia coli K-12 by the dam and dcm enzymes.

Journal of bacteriology ·Vol. 147 ·No. 1 ·1981-07-00 ·Pages 259-61

Dreiseikelmann B, Wackernagel W

Abstract

Restriction analysis of plasmid pHV14 deoxyribonucleic acid isolated from Escherichia coli K-12, Bacillus subtilis, and staphylococcus aureus with restriction endonucleases MboI, Sau3AI, and EcoRII was used to study the methylation of those nucleotide sequences which in E. coli contain the major portions of N6-methyladenine and 5-methylcytosine. The results showed that neither B. subtilis nor S. aureus methylates deoxyribonucleic acid at the same sites and nucleotides which are recognized and methylated by dam and dcm enzymes in E. coli K-12.

MeSH Terms
Adenine/metabolism Bacillus subtilis/metabolism Base Sequence Cytosine/metabolism DNA (Cytosine-5-)-Methyltransferases/metabolism DNA Restriction Enzymes DNA, Bacterial/metabolism Escherichia coli/metabolism Methylation Methyltransferases/metabolism Staphylococcus aureus/metabolism
Chemicals
DNA, Bacterial Cytosine Methyltransferases DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dreiseikelmann B
Wackernagel W
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.1.259-261.1981
Published
1981-07-00
Pages
259-61
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216033
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.147.1.259-261.1981 PMC Full Text View journal details
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