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PMID: 6273315 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunization with major outer membrane protein (porin) preparations in experimental murine salmonellosis: effect of lipopolysaccharide.

Infection and immunity ·Vol. 34 ·No. 2 ·1981-11-00 ·Pages 328-32

Kuusi N, Nurminen M, Saxén H, Mäkelä PH

Abstract

A crude major outer membrane (porin) preparation, obtained from a rough strain of Salmonella typhimurium earlier shown to be protective both in active and passive immunization of mice against challenge with smooth S. typhimurium, was further purified. Removal of the main impurities, lipopolysaccharide (LPS) and lipoprotein, was accompanied by loss of protective capacity in passive immunization experiments. Reconstitution with rough LPS restored the protective capacity. Protection was, however, concluded not to be due to anti-LPS, because a large fraction of the anti-LPS antibodies could be removed from the protective rabbit antiserum with an LPS immunosorbent without loss of protection.

MeSH Terms
Animals Bacterial Proteins/immunology,isolation & purification Immunization, Passive Lipopolysaccharides/physiology Membrane Proteins/immunology,isolation & purification Mice Porins Salmonella Infections, Animal/immunology Salmonella typhimurium/immunology
Chemicals
Bacterial Proteins Lipopolysaccharides Membrane Proteins Porins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kuusi N
Nurminen M
Saxén H
Mäkelä P H
References (28)
28 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.34.2.328-332.1981
Published
1981-11-00
Pages
328-32
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350868
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.34.2.328-332.1981 PMC Full Text View journal details
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