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PMID: 6285311 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of a site of psoralen crosslinking in E. coli 16S ribosomal RNA.

Nucleic acids research ·Vol. 10 ·No. 9 ·1982-05-11 ·Pages 2839-49

Turner S, Thompson JF, Hearst JE, Noller HF

Abstract

We have developed a 2-dimensional gel method for identification of RNA sequences crosslinked by the intercalative drug 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). This method is being used to localize such sites in E. coli ribosomal RNA. We report here the identification of a site for HMT crosslinking within positions 434 and 497 of 16S rRNA. We suggest a likely site for HMT intercalation, in which residues U548 and U473 become crosslinked via the drug.

MeSH Terms
Base Sequence Chemical Phenomena Chemistry Electrophoresis, Polyacrylamide Gel Endonucleases Escherichia coli Furocoumarins Molecular Weight Nucleic Acid Conformation RNA, Ribosomal Ribonuclease T1 Ribonuclease, Pancreatic Ribonucleases Trioxsalen/analogs & derivatives
Chemicals
Furocoumarins RNA, Ribosomal hydroxymethyltrioxsalen Endonucleases Ribonucleases Ribonuclease T1 Ribonuclease, Pancreatic Trioxsalen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Turner S
Thompson J F
Hearst J E
Noller H F
References (40)
40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.9.2839
Published
1982-05-11
Pages
2839-49
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320659
Subset
IM
Grants
NIGMS NIH HHS · GM-11180 · United States
NIGMS NIH HHS · GM-17129 · United States
External Links
PubMed source DOI: 10.1093/nar/10.9.2839 PMC Full Text View journal details
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