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PMID: 6319965 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Deletion of 60 kilobase pairs of DNA from the terC region of the chromosome of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 193 ·No. 2 ·1984-00-00 ·Pages 263-8

Henson JM, Kopp B, Kuempel PL

Abstract

The terminus of replication (terC) of the chromosome of Escherichia coli is located between the rac (min 30.0) and manA (min 35.7) loci, presumably close to the trg (min 31.4) locus. We have used a strain containing lambda reverse (min 30.0) and trg-2::Tn10 (min 31.4) to obtain deletions of the entire 60 kilobase pair region that separates these elements. Strains harboring these deletions possessed fusion fragments that contained DNA homologous to both lambda reverse and trg region DNA. In addition, chromosomal DNA normally present between min 30.0 and min 31.4 was absent in these strains. The strains had no readily apparent mutant phenotype, which demonstrates that this large region of DNA is not essential for normal growth.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Chromosome Deletion Chromosomes, Bacterial DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics Protein Biosynthesis Transduction, Genetic
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Henson J M
Kopp B
Kuempel P L
References (30)
30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330678
Published
1984-00-00
Pages
263-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330678
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