Evidence for variation of supercoil densities among simian virus 40 nucleoprotein complexes and for higher supercoil density in replicating complexes.
The distribution of DNA topoisomers in intracellular simian virus 40 DNA was analyzed by gel electrophoresis. The results suggested that DNA extracted from 70S chromatin had a different superhelical density distribution as compared with the DNA obtained from virions or virion assembly intermediates. The heterogeneity of simian virus 40 viral DNA superhelical density at a late time after infection was partly due to increased virion production and partly due to the intrinsic heterogeneity of the superhelical density of DNA extracted from virions. Using two-dimensional gel electrophoretic analysis we also showed that simian virus 40 DNA templates used for DNA replication have a higher average superhelical density than the bulk of intracellular viral DNA.
19 references, click to expand
-
Selective extraction of polyoma DNA from infected mouse cell cultures.J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
-
Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.EMBO J. 1982;1(11):1353-8 PMID: 6327265
-
Conformational fluctuations of DNA helix.Proc Natl Acad Sci U S A. 1975 Nov;72(11):4275-9 PMID: 172901
-
Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.Proc Natl Acad Sci U S A. 1975 Dec;72(12):4876-80 PMID: 174079
-
The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.Nucleic Acids Res. 1977;4(5):1183-205 PMID: 197488
-
Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells.J Virol. 1979 Feb;29(2):612-23 PMID: 219250
-
Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly.J Virol. 1979 Jul;31(1):199-208 PMID: 228054
-
Terminal stages of SV40 DNA replication proceed via multiply intertwined catenated dimers.Cell. 1980 Aug;21(1):103-14 PMID: 6250706
-
Intracellular forms of simian virus 40 nucleoprotein complexes. III. Study of histone modifications.J Virol. 1980 Nov;36(2):353-60 PMID: 6253661
-
DNA wrapping in nucleosomes. The linking number problem re-examined.Nucleic Acids Res. 1980 Oct 24;8(20):4803-20 PMID: 6255433
-
Structure of chromatin and the linking number of DNA.Proc Natl Acad Sci U S A. 1981 Mar;78(3):1461-5 PMID: 6940168
-
DNA supercoiling: another level for regulating gene expression.Cell. 1981 Jun;24(3):599-600 PMID: 6265097
-
The dependence of the linking number of a circular minichromosome upon the shape and the orientation of its nucleosomes.FEBS Lett. 1981 Jul 20;130(1):43-6 PMID: 7286225
-
The helical periodicity of DNA on the nucleosome.Nucleic Acids Res. 1981 Sep 11;9(17):4267-83 PMID: 6272202
-
Prereplicative events involving simian virus 40 DNA in permissive cells.J Virol. 1982 Jan;41(1):237-43 PMID: 6283116
-
Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion.J Virol. 1982 Nov;44(2):603-9 PMID: 6292520
-
The path of DNA in the nucleosome.Cell. 1982 Jul;29(3):724-6 PMID: 7151166
-
Polyoma virus minichromosomes: characterization of the products of in vitro DNA synthesis.J Virol. 1983 Feb;45(2):585-93 PMID: 6300422
-
Folding of the DNA double helix in chromatin-like structures from simian virus 40.Proc Natl Acad Sci U S A. 1975 May;72(5):1843-7 PMID: 168578
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