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PMID: 6255433 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA wrapping in nucleosomes. The linking number problem re-examined.

Nucleic acids research ·Vol. 8 ·No. 20 ·1980-10-24 ·Pages 4803-20

Stein A

Abstract

Chromatin was assembled in vitro from relaxed closed circular DNA (SV40) and core histones at histone to DNA ratios of 0.2 to 0.3 (g/g) and incubated with topoisomerase I to relax supercoils in DNA regions not constrained by protein. Addition of histones H1 + H5 to the chromatin at an ionic strength of 0.1 M, in the presence of the solubilizing agent, polyglutamic acid, and topoisomerase I, increased the magnitude of the DNA linking number change, relative to protein-free DNA. No change in the linking number distribution occurred for relaxed protein-free DNA under these conditions. Control experiments indicated that the increase in the absolute value of the DNA linking number change in the chromatin could not be attributed to an increase in the number of nucleosomes per DNA molecule. These data suggest a solution to the linking number problem associated with models of chromatin structure.

MeSH Terms
Animals Base Composition Chickens DNA, Circular/analysis DNA, Superhelical/analysis DNA, Viral/analysis Erythrocytes/analysis Histones/analysis Nucleosomes/ultrastructure Simian virus 40/analysis
Chemicals
DNA, Circular DNA, Superhelical DNA, Viral Histones Nucleosomes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Stein A
References (37)
37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.20.4803
Published
1980-10-24
Pages
4803-20
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324389
Subset
IM
Grants
NIGMS NIH HHS · 1 RO1 GM27719-01 · United States
External Links
PubMed source DOI: 10.1093/nar/8.20.4803 PMC Full Text View journal details
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