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PMID: 6328410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A minor class of 5S rRNA genes in Saccharomyces cerevisiae X2180-1B, one member of which lies adjacent to a Ty transposable element.

Nucleic acids research ·Vol. 12 ·No. 10 ·1984-05-25 ·Pages 4083-96

Piper PW, Lockheart A, Patel N

Abstract

In Saccharomyces cerevisiae the majority of the genes for 5S rRNA lie within a 9kb rDNA sequence that is present as 100-200 tandemly-repeated copies on Chromosome XII. Following our observations that about 10% of yeast 5S rRNA exists as minor variant sequences, we screened a collection of yeast DNA fragments cloned in lambda gt for 5S rRNA genes whose flanking sequences differed from those adjacent to 5S rRNA genes of the rDNA repeat. Three variant 5S rRNA genes were isolated on the basis of such dissimilarity to rDNA repeat sequences. They display a remarkable conservation of their DNA in the vicinity of the 5S coding region, and are examples of a minor form of 5S rRNA coding sequence present in a small number of copies in the yeast genome. These variant sequences appear to be transcribed as efficiently as 5S rRNA genes of the rDNA repeat. In one of our isolates of the variant sequence a Ty transposable element is inserted 145bp upstream of the initiation point for 5S rRNA synthesis.

MeSH Terms
Base Sequence DNA/genetics DNA Restriction Enzymes DNA Transposable Elements DNA, Recombinant/metabolism DNA, Ribosomal Genes, Fungal Molecular Weight Nucleic Acid Hybridization Plasmids RNA, Ribosomal/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA Transposable Elements DNA, Recombinant DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Piper P W
Lockheart A
Patel N
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44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.10.4083
Published
1984-05-25
Pages
4083-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318818
Subset
IM
Databases
GENBANK
X00602, X00603, X00604
External Links
PubMed source DOI: 10.1093/nar/12.10.4083 PMC Full Text View journal details
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