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PMID: 6572011 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A seven-base-pair deletion in an intron of the albumin gene of analbuminemic rats.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 1 ·1983-01-00 ·Pages 95-9

Esumi H, Takahashi Y, Sato S, Nagase S, Sugimura T

Abstract

Analbuminemic rats, which genetically lack serum albumin, have a mutation affecting albumin mRNA processing. Serum albumin genes were cloned from analbuminemic and normal parental Sprague-Dawley rats. Structural analyses of the two albumin genes showed that the gene from analbuminemic rats had a seven-base-pair deletion in an intron. The deletion extended from base 5 to base 11 from the 5' end of intron HI of the albumin gene. This deletion converted the sequence, G-T-A-G-G-T, which is normally located at the 5' end of intron HI, to G-T-A-G-C-G. RNA blot hybridization of analbuminemic and normal rat liver nuclear RNA using a DNA fragment containing the intron HI as a probe showed that this intron sequence persisted in albumin mRNA precursors of analbuminemic rats.

MeSH Terms
Animals Base Sequence Chromosome Deletion Cloning, Molecular Gene Expression Regulation Genes RNA Splicing Rats Rats, Mutant Strains/genetics Serum Albumin/deficiency,genetics
Chemicals
Serum Albumin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Esumi H
Takahashi Y
Sato S
Nagase S
Sugimura T
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.1.95
Published
1983-01-00
Pages
95-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393316
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.1.95 PMC Full Text View journal details
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