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PMID: 6790913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Integration and excision of a plasmid in Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 182 ·No. 1 ·1981-00-00 ·Pages 99-105

Galizzi A, Scoffone F, Milanesi G, Albertini AM

Abstract

We have studied the behaviour in Bacillus subtilis of a plasmid (pPV21) carrying the thymidylate synthetase gene of phage phi3T (thyP3). The plasmid can transform efficiently the competent cells of all the strains tested. Polyethylene glycol (PEG)-mediated protoplast transformation is efficient only for recE, recD or recF mutants. When present in recombination proficient strains, the plasmid can be integrated into the chromosome, primarily at the thyA locus. This has been shown by genetic mapping and by blot-hybridization. A second less efficient site is at (or near to) the attachment site of phage phi3T. Excision of the plasmid restores the EcoRI restriction pattern of the parental DNA, although with the loss of the defective thyA endogenotic allele and the retention of the thyP exogenotic gene.

MeSH Terms
Bacillus subtilis/genetics Chromosomes, Bacterial/physiology DNA, Recombinant Nucleic Acid Hybridization Plasmids Recombination, Genetic Thymidylate Synthase/genetics Transformation, Genetic
Chemicals
DNA, Recombinant Thymidylate Synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Galizzi A
Scoffone F
Milanesi G
Albertini A M
References (24)
24 references, click to expand
  1. DNA cloning in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1433-6 PMID: 418413
  2. Construction of biologically functional bacterial plasmids in vitro.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
  3. Transducing particles of PBS 1.
    Virology. 1968 Dec;36(4):639-45 PMID: 4972797
  4. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  5. Temperate Bacillus subtilis bacteriophage phi 3T: chromosomal attachment site and comparison with temperate bacteriophages phi 105 and SPO2.
    J Virol. 1977 Feb;21(2):522-9 PMID: 401899
  6. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  7. Infection of Bacillus subtilis with phenol-extracted bacteriophages.
    Virology. 1962 Apr;16:452-9 PMID: 14493621
  8. Construction of a colony bank of E. coli containing hybrid plasmids representative of the Bacillus subtilis 168 genome. Expression of functions harbored by the recombinant plasmids in B. subtilis.
    Mol Gen Genet. 1979 Oct 3;176(2):239-45 PMID: 119126
  9. Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4145-9 PMID: 825858
  10. Transformation and transduction in recombination-defective mutants of Bacillus subtilis.
    J Bacteriol. 1967 Jun;93(6):1925-37 PMID: 4960898
  11. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  12. The Bacillus subtilis chromosome.
    Microbiol Rev. 1980 Mar;44(1):57-82 PMID: 6774224
  13. Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.
    J Biol Chem. 1970 Jan 10;245(1):39-45 PMID: 4904090
  14. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680-2 PMID: 404641
  15. Recombinant plasmids capable to replication in B. subtilis and E. coli.
    Mol Gen Genet. 1978 Jun 1;162(1):59-67 PMID: 97514
  16. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  17. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  18. Construction and properties of chimeric plasmids in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1428-32 PMID: 418412
  19. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.
    Biochim Biophys Acta. 1963 Aug 20;72:619-29 PMID: 14071565
  20. Mechanism of integrating foreign DNA during transformation of Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3664-8 PMID: 99740
  21. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
  22. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
    Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
  23. Genetic recombination during transformation in Bacillus subtilis: appearance of a deoxyribonucleic acid methylase.
    J Bacteriol. 1979 Jul;139(1):270-9 PMID: 110783
  24. Mapping a cloned gene under sporulation control by inserttion of a drug resistance marker into the Bacillus subtilis chromosome.
    J Bacteriol. 1980 Apr;142(1):90-8 PMID: 6768719
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00422774
Published
1981-00-00
Pages
99-105
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00422774
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