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PMID: 6973367 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA repair after ultraviolet irradiation of ICR 2A frog cells. Pyrimidine dimers are long acting blocks to nascent DNA synthesis.

Biophysical journal ·Vol. 31 ·No. 2 ·1980-08-00 ·Pages 195-205

Rosenstein BS, Setlow RB

Abstract

The ability of ICR 2A frog cells to repair DNA damage induced by ultraviolet irradiation was examined. These cells are capable of photoreactivation but are nearly totally deficient in excision repair. They have the ability to convert the small molecule weight DNA made after irradiation into large molecules but do not show an enhancement in this process when the UV dose is delivered in two separate exposures separated by a 3- or 24-h incubation. Total DNA synthesis is depressed and low molecular weight DNA continues to be synthesized during pulse-labeling as long as 48 h after irradiation. The effects of pyrimidine dimer removal through exposure of UV irradiated cells to photoreactivating light indicate that dimers act as the critical lesions blocking DNA synthesis.

MeSH Terms
Animals Bromodeoxyuridine/pharmacology Cell Line DNA/biosynthesis,radiation effects DNA Repair Haploidy Photolysis Pyrimidine Dimers/metabolism Rana pipiens/embryology Ultraviolet Rays
Chemicals
Pyrimidine Dimers DNA Bromodeoxyuridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rosenstein B S
Setlow R B
References (19)
19 references, click to expand
  1. The chemical nature of photoreactivable lesions in DNA.
    Proc Natl Acad Sci U S A. 1965 Jun;53(6):1430-6 PMID: 5324621
  2. Single strand interruptions in DNA and the effects of caffeine in Chinese hamster cells irradiated with ultraviolet light.
    Biochem Biophys Res Commun. 1969 Jul 23;36(2):203-8 PMID: 5816421
  3. Stable haploid cultured cell lines from frog embryos.
    Proc Natl Acad Sci U S A. 1970 Feb;65(2):337-44 PMID: 5263768
  4. Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA.
    Proc Natl Acad Sci U S A. 1969 Nov;64(3):1035-41 PMID: 5264135
  5. Two forms of repair of DNA in mammalian cells following irradiation.
    Biophys J. 1970 Mar;10(3):237-45 PMID: 5461576
  6. Normal and defective repair of damaged DNA in human cells: a sensitive assay utilizing the photolysis of bromodeoxyuridine.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):708-12 PMID: 5279512
  7. Deoxyribonucleic acid synthesis in ultraviolet-light-irradiated Chinese hamster cells.
    Biophys J. 1971 Mar;11(3):295-301 PMID: 5103274
  8. DNA chain elongation and joining in normal human and xeroderma pigmentosum cells after ultraviolet irradiation.
    Biophys J. 1972 Sep;12(9):1183-91 PMID: 5056962
  9. A rapid and sensitive assay for pyrimidine dimers in DNA.
    Anal Biochem. 1973 May;53(1):168-76 PMID: 4351118
  10. Recovery of the ability to synthesize DNA in segments of normal size at long times after ultraviolet irradiation of human cells.
    Biophys J. 1973 Dec;13(12):1265-75 PMID: 4761575
  11. Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens.
    Cancer Res. 1974 Dec;34(12):3318-25 PMID: 4371955
  12. Postreplication repair of DNA in chick cells: studies using photoreactivation.
    Biochim Biophys Acta. 1975 Aug 21;402(2):179-87 PMID: 1236746
  13. Enhancement of postreplication repair in Chinese hamster cells.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2396-400 PMID: 1065893
  14. Semi-conservative deoxyribonucleic acid synthesis in unirradiated and ultraviolet-irradiated xeroderma pigmentosum and normal human skin fibroblasts.
    Mutat Res. 1977 Mar;42(3):433-42 PMID: 854043
  15. Enhancement of postreplication repair in ultraviolet-light-irradiated Chinese hamster cells by irradiation in G2 or S-phase.
    Biophys J. 1978 Jul;23(1):71-8 PMID: 667307
  16. Does ultraviolet light enhance postreplication repair in mammalian cells?
    Nature. 1978 Sep 21;275(5677):243-5 PMID: 692701
  17. Isolation of UV-sensitive clones from a haploid frog cell line.
    Somatic Cell Genet. 1979 Jan;5(1):117-28 PMID: 432753
  18. Survival and DNA repair in ultraviolet-irradiated haploid and diploid cultured frog cells.
    Mutat Res. 1979 Sep;62(2):325-39 PMID: 315517
  19. Use of the dye Hoechst 33258 in a modification of the bromodeoxyuridine photolysis technique for the analysis of DNA repair.
    Photochem Photobiol. 1980 Mar;31(3):215-22 PMID: 6154299
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(80)85050-8
Published
1980-08-00
Pages
195-205
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1328777
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(80)85050-8 PMC Full Text View journal details
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