Home LiteratureArticle Details
PMID: 7595209 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The cytoplasmic domains of immunoglobulin (Ig) alpha and Ig beta can independently induce the precursor B cell transition and allelic exclusion.

The Journal of experimental medicine ·Vol. 182 ·No. 5 ·1995-11-01 ·Pages 1389-94

Papavasiliou F, Jankovic M, Suh H, Nussenzweig MC

Abstract

In mature B cells, signals transduced through membrane immunoglobulin (Ig) produce cellular activation, yet the same receptor can also mediate deletion and silencing of autoreactive B cells. In addition, Ig expression during the antigen-independent phase of B cell development regulates the precursor B (pre-B) cell transition and allelic exclusion. To account for the diverse regulatory functions induced by membrane Ig, it has been proposed that individual receptor components have independent physiologic activities. Here we establish a role for Ig alpha in the pre-B cell transition and allelic exclusion. We find that the cytoplasmic domain of Ig alpha contains sufficient information to trigger both of these antigen-independent events. Direct comparisons of the cytoplasmic domains of Ig alpha and Ig beta show that the two are indistinguishable in the induction of the pre-B cell transition and allelic exclusion. Our experiments suggest that, despite the reported differences in certain biochemical assays, Ig alpha and Ig beta have redundant functions in the developing B cell.

MeSH Terms
Alleles Animals Antigens, CD/chemistry,physiology B-Lymphocytes/cytology CD79 Antigens Cell Differentiation Clonal Deletion Genes, Immunoglobulin H-2 Antigens/immunology Hematopoietic Stem Cells/cytology Immunoglobulin Heavy Chains/physiology Immunoglobulin Light Chains/physiology Mice Mice, Transgenic Peptide Fragments/pharmacology Protein Structure, Tertiary Receptors, Antigen, B-Cell/chemistry,genetics,physiology Recombinant Fusion Proteins/genetics,physiology Self Tolerance Signal Transduction Transgenes src Homology Domains/physiology
Chemicals
Antigens, CD CD79 Antigens Cd79a protein, mouse Cd79b protein, mouse H-2 Antigens H-2K(K) antigen Immunoglobulin Heavy Chains Immunoglobulin Light Chains Peptide Fragments Receptors, Antigen, B-Cell Recombinant Fusion Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Papavasiliou F
Laboratory of Molecular Immunology, Rockefeller University, New York, USA.
Jankovic M
Suh H
Nussenzweig M C
References (44)
44 references, click to expand
  1. Signal transduction by immunoglobulin is mediated through Ig alpha and Ig beta.
    J Exp Med. 1993 Sep 1;178(3):1049-55 PMID: 7688784
  2. Receptor editing in self-reactive bone marrow B cells.
    J Exp Med. 1993 Apr 1;177(4):1009-20 PMID: 8459201
  3. The alpha/beta sheath and its cytoplasmic tyrosines are required for signaling by the B-cell antigen receptor but not for capping or for serine/threonine-kinase recruitment.
    Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):474-8 PMID: 8290550
  4. Signal transduction by lymphocyte antigen receptors.
    Cell. 1994 Jan 28;76(2):263-74 PMID: 8293463
  5. Biochemistry of B lymphocyte activation.
    Adv Immunol. 1994;55:221-95 PMID: 8304234
  6. Different patterns of calcium signaling triggered through two components of the B lymphocyte antigen receptor.
    J Biol Chem. 1994 Mar 4;269(9):6491-7 PMID: 7509805
  7. Roles of IgH and L chains and of surrogate H and L chains in the development of cells of the B lymphocyte lineage.
    Annu Rev Immunol. 1994;12:209-25 PMID: 8011281
  8. Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice.
    Genes Dev. 1994 May 1;8(9):1030-42 PMID: 7926785
  9. Influence of immunoglobulin heavy- and light-chain expression on B-cell differentiation.
    Genes Dev. 1994 May 1;8(9):1043-57 PMID: 7926786
  10. The B-cell antigen receptor complex: structure and signal transduction.
    Immunol Today. 1994 Sep;15(9):393-9 PMID: 7945780
  11. Role of TCR zeta chain in T cell development and selection.
    Science. 1994 Nov 11;266(5187):1047-50 PMID: 7526464
  12. The site and stage of anti-DNA B-cell deletion.
    Nature. 1995 Jan 19;373(6511):252-5 PMID: 7816141
  13. The role of Ig beta in precursor B cell transition and allelic exclusion.
    Science. 1995 Apr 21;268(5209):408-11 PMID: 7716544
  14. CD3 epsilon and CD3 zeta cytoplasmic domains can independently generate signals for T cell development and function.
    Immunity. 1995 Apr;2(4):401-11 PMID: 7719942
  15. Reconstitution of Syk function by the ZAP-70 protein tyrosine kinase.
    Immunity. 1995 May;2(5):485-92 PMID: 7538440
  16. The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes.
    J Biol Chem. 1981 May 25;256(10):5116-20 PMID: 6262318
  17. Introduction of a mu immunoglobulin gene into the mouse germ line: specific expression in lymphoid cells and synthesis of functional antibody.
    Cell. 1984 Oct;38(3):647-58 PMID: 6091894
  18. Role of an RNA cleavage/poly(A) addition site in the production of membrane-bound and secreted IgM mRNA.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8658-62 PMID: 3936040
  19. Germ-line transformation of mice.
    Annu Rev Genet. 1986;20:465-99 PMID: 3545063
  20. B lymphocytes may escape tolerance by revising their antigen receptors.
    J Exp Med. 1993 Apr 1;177(4):1165-73 PMID: 8459210
  21. Analysis of the interaction of ZAP-70 and syk protein-tyrosine kinases with the T-cell antigen receptor by plasmon resonance.
    Proc Natl Acad Sci U S A. 1995 May 23;92(11):5106-10 PMID: 7761456
  22. Allelic exclusion in transgenic mice that express the membrane form of immunoglobulin mu.
    Science. 1987 May 15;236(4803):816-9 PMID: 3107126
  23. Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic mice.
    Nature. 1988 Aug 25;334(6184):676-82 PMID: 3261841
  24. B29: a member of the immunoglobulin gene superfamily exclusively expressed on beta-lineage cells.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6890-4 PMID: 3137575
  25. Feedback inhibition of immunoglobulin gene rearrangement by membrane mu, but not by secreted mu heavy chains.
    J Exp Med. 1988 Oct 1;168(4):1363-81 PMID: 3139821
  26. A novel 34-kd protein co-isolated with the IgM molecule in surface IgM-expressing cells.
    EMBO J. 1988 Nov;7(11):3451-6 PMID: 3264786
  27. B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties.
    EMBO J. 1988 Nov;7(11):3457-64 PMID: 2463161
  28. Clonal deletion of B lymphocytes in a transgenic mouse bearing anti-MHC class I antibody genes.
    Nature. 1989 Feb 9;337(6207):562-6 PMID: 2783762
  29. Antigen receptor tail clue.
    Nature. 1989 Mar 30;338(6214):383-4 PMID: 2927501
  30. Cellular immunity to HIV activated by CD4 fused to T cell or Fc receptor polypeptides.
    Cell. 1991 Mar 8;64(5):1037-46 PMID: 1900456
  31. The cytoplasmic domain of the T cell receptor zeta chain is sufficient to couple to receptor-associated signal transduction pathways.
    Cell. 1991 Mar 8;64(5):891-901 PMID: 1705867
  32. A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin mu chain gene.
    Nature. 1991 Apr 4;350(6317):423-6 PMID: 1901381
  33. A transgenic model of autoimmune hemolytic anemia.
    J Exp Med. 1992 Jan 1;175(1):71-9 PMID: 1730928
  34. The T cell receptor/CD3 complex is composed of at least two autonomous transduction modules.
    Cell. 1992 Jan 10;68(1):83-95 PMID: 1531041
  35. Targeted disruption of mu chain membrane exon causes loss of heavy-chain allelic exclusion.
    Nature. 1992 Mar 12;356(6365):154-6 PMID: 1545868
  36. RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
    Cell. 1992 Mar 6;68(5):855-67 PMID: 1547487
  37. RAG-1-deficient mice have no mature B and T lymphocytes.
    Cell. 1992 Mar 6;68(5):869-77 PMID: 1547488
  38. Chromosomal position of rearranging gene segments influences allelic exclusion in transgenic mice.
    Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2205-8 PMID: 1372438
  39. Activation of T cells by a tyrosine kinase activation domain in the cytoplasmic tail of CD3 epsilon.
    Science. 1992 Jan 3;255(5040):79-82 PMID: 1532456
  40. Antigen receptors on B lymphocytes.
    Annu Rev Immunol. 1992;10:97-121 PMID: 1591006
  41. Transmembrane signaling: the joy of aggregation.
    J Immunol. 1992 Sep 1;149(5):1477-87 PMID: 1324276
  42. The B cell antigen receptor complex: association of Ig-alpha and Ig-beta with distinct cytoplasmic effectors.
    Science. 1992 Oct 2;258(5079):123-6 PMID: 1439759
  43. Differential signaling through the Ig-alpha and Ig-beta components of the B cell antigen receptor.
    Eur J Immunol. 1993 Apr;23(4):911-6 PMID: 7681402
  44. Signaling-defective mutants of the B lymphocyte antigen receptor fail to associate with Ig-alpha and Ig-beta/gamma.
    J Biol Chem. 1993 Dec 5;268(34):25776-9 PMID: 8245014
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.182.5.1389
Published
1995-11-01
Pages
1389-94
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192193
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.182.5.1389 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2