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PMID: 7739537 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vivo association of E2F and DP family proteins.

Molecular and cellular biology ·Vol. 15 ·No. 5 ·1995-05-00 ·Pages 2536-46

Wu CL, Zukerberg LR, Ngwu C, Harlow E, Lees JA

Abstract

The mammalian transcription factor E2F plays an important role in regulating the expression of genes that are required for passage through the cell cycle. This transcriptional activity is inhibited by association with the retinoblastoma tumor suppressor protein (pRB) or its relatives p107 and p103. The first cDNA from the E2F family to be cloned was designated E2F-1, and multiple E2F family members have now been identified. They bind to DNA as heterodimers, interacting with proteins known as DP. Here we demonstrate that DP is also a family of polypeptides with at least two members (hDP-1 and hDP-2). Both hDP-1 and hDP-2 bind to all E2F family members in vivo, and each complex is capable of activating transcription. However, the various E2F/DP complexes display strong differences in the ability to bind to either pRB or p107 in vivo, and the specificity of pRB or p107 binding is mediated by the E2F subunit.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins Cell Cycle Proteins Cell Line DNA/metabolism DNA, Complementary/genetics DNA-Binding Proteins/genetics,metabolism Drosophila Proteins E2F Transcription Factors E2F1 Transcription Factor Gene Expression Humans Mice Molecular Sequence Data Protein Binding RNA, Messenger/genetics,metabolism Retinoblastoma-Binding Protein 1 Trans-Activators/genetics,metabolism Transcription Factor DP1 Transcription Factors/genetics,metabolism Transcriptional Activation
Chemicals
Arid4a protein, mouse Carrier Proteins Cell Cycle Proteins DNA, Complementary DNA-Binding Proteins Dp transcription factor, Drosophila Drosophila Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human E2f1 protein, mouse RNA, Messenger Retinoblastoma-Binding Protein 1 Tfdp1 protein, mouse Trans-Activators Transcription Factor DP1 Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wu C L
Massachusetts General Hospital Cancer Center, Charlestown 02129, USA.
Zukerberg L R
Ngwu C
Harlow E
Lees J A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.5.2536
Published
1995-05-00
Pages
2536-46
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230484
Subset
IM
Databases
GENBANK
L40386
External Links
PubMed source DOI: 10.1128/MCB.15.5.2536 PMC Full Text View journal details
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