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PMID: 7751283 Published · ppublish English Comparative Study Journal Article

Characterization of the umu-complementing operon from R391.

Journal of bacteriology ·Vol. 177 ·No. 10 ·1995-05-00 ·Pages 2737-43

Kulaeva OI, Wootton JC, Levine AS, Woodgate R

Abstract

In addition to conferring resistances to antibiotics and heavy metals, certain R factors carry genes involved in mutagenic DNA repair. These plasmid-encoded genes are structurally and functionally related to the chromosomally encoded umuDC genes of Escherichia coli and Salmonella typhimurium. Three such plasmid operons, mucAB, impCAB, and samAB, have been characterized at the molecular level. Recently, we have identified three additional umu-complementing operons from IncJ plasmid R391 and IncL/M plasmids R446b and R471a. We report here the molecular characterization of the R391 umu-complementing operon. The nucleotide sequence of the minimal R plasmid umu-complementing (rum) region revealed an operon of two genes, rumA(R391) and rumB(R391), with an upstream regulatory signal strongly resembling LexA-binding sites. Phylogenetic analysis revealed that the RumAB(R391) proteins are approximately equally diverged in sequence from the chromosomal UmuDC proteins and the other plasmid-encoded Umu-like proteins and represent a new subfamily. Genetic characterization of the rumAB(R391) operon revealed that in recA+ and recA1730 backgrounds, the rumAB(R391) operon was phenotypically indistinguishable from mucAB. In contrast, however, the rumAB(R391) operon gave levels of mutagenesis that were intermediate between those given by mucAB and umuDC in a recA430 strain. The latter phenotype was shown to correlate with the reduced posttranslational processing of the RumA(R391) protein to its mutagenically active form, RumA'(R391). Thus, the rumAB(R391) operon appears to possess characteristics that are reminiscent of both chromosome and plasmid-encoded umu-like operons.

Related Genes
mucA mucB rumA rumB umuC umuD
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA Repair/genetics DNA-Directed DNA Polymerase Escherichia coli/genetics,radiation effects Escherichia coli Proteins Genes, Bacterial/genetics Genetic Complementation Test Molecular Sequence Data Mutagenesis/genetics Operon/genetics Phylogeny R Factors/genetics Regulatory Sequences, Nucleic Acid/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Ultraviolet Rays
Chemicals
Bacterial Proteins Escherichia coli Proteins MucA protein, Bacteria MucB protein, Bacteria RumA protein, Bacteria RumB protein, E coli UmuC protein, E coli DNA-Directed DNA Polymerase UmuD protein, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kulaeva O I
Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892-2725, USA.
Wootton J C
Levine A S
Woodgate R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.10.2737-2743.1995
Published
1995-05-00
Pages
2737-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176944
Subset
IM
Databases
GENBANK
U13633
External Links
PubMed source DOI: 10.1128/jb.177.10.2737-2743.1995 PMC Full Text View journal details
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