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PMID: 7816623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The yeast BDF1 gene encodes a transcription factor involved in the expression of a broad class of genes including snRNAs.

Nucleic acids research ·Vol. 22 ·No. 24 ·1994-12-11 ·Pages 5332-40

Lygerou Z, Conesa C, Lesage P, Swanson RN, Ruet A, Carlson M, Sentenac A, Séraphin B

Abstract

While screening for genes that affect the synthesis of yeast snRNPs, we identified a thermosensitive mutant that abolishes the production of a reporter snRNA at the non-permissive temperature. This mutant defines a new gene, named BDF1. In a bdf1-1 strain, the reporter snRNA synthesized before the temperature shift remains stable at the non-permissive temperature. This demonstrates that the BDF1 gene affects the synthesis rather than the stability of the reporter snRNA and suggests that the BDF1 gene encodes a transcription factor. BDF1 is present in single copy on yeast chromosome XII, and is important for normal vegetative growth but not essential for cell viability. bdf1 null mutants share common phenotypes with several mutants affecting general transcription and are defective in snRNA production. BDF1 encodes a protein of 687 amino-acids containing two copies of the bromodomain, a motif also present in other transcription factors as well as a new conserved domain, the ET domain, also present in Drosophila and human proteins.

Related Genes
BDF1
MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Chromosomes, Fungal Cloning, Molecular Conserved Sequence Gene Dosage Gene Expression Regulation, Fungal/physiology Genes, Fungal/genetics Genes, Regulator/genetics Molecular Sequence Data Mutation RNA, Small Nuclear/biosynthesis Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Analysis, DNA Temperature Transcription Factors/genetics,physiology
Chemicals
BDF1 protein, S cerevisiae RNA, Small Nuclear Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lygerou Z
EMBL, Heidelberg, Germany.
Conesa C
Lesage P
Swanson R N
Ruet A
Carlson M
Sentenac A
Séraphin B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.24.5332
Published
1994-12-11
Pages
5332-40
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332079
Subset
IM
Grants
NIGMS NIH HHS · GM47259 · United States
Databases
GENBANK
Z18944
External Links
PubMed source DOI: 10.1093/nar/22.24.5332 PMC Full Text View journal details
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