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PMID: 1535687 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes.

Molecular and cellular biology ·Vol. 12 ·No. 7 ·1992-07-00 ·Pages 3247-61

Murphy S, Yoon JB, Gerster T, Roeder RG

Abstract

The promoters of both RNA polymerase II- and RNA polymerase III-transcribed small nuclear RNA (snRNA) genes contain an essential and highly conserved proximal sequence element (PSE) approximately 55 bp upstream from the transcription start site. In addition, the upstream enhancers of all snRNA genes contain binding sites for octamer-binding transcription factors (Octs), and functional studies have indicated that the PSE and octamer elements work cooperatively. The present study has identified and characterized a novel transcription factor (designated PTF) which specifically binds to the PSE sequence of both RNA polymerase II- and RNA polymerase III-transcribed snRNA genes. PTF binding is markedly potentiated by Oct binding to an adjacent octamer site. This potentiation is effected by Oct-1, Oct-2, or the conserved POU domain of these factors. In agreement with these results and despite the independent binding of Octs to the promoter, PTF and Oct-1 enhance transcription from the 7SK promoter in an interdependent manner. Moreover, the POU domain of Oct-1 is sufficient for significant in vitro activity in the presence of PTF. These results suggest that essential activation domains reside in PTF and that the potentiation of PTF binding by Octs plays a key role in the function of octamer-containing snRNA gene enhancers.

MeSH Terms
Base Sequence Chromosome Mapping DNA Probes/metabolism DNA-Binding Proteins/genetics Enhancer Elements, Genetic/genetics Host Cell Factor C1 Humans Molecular Sequence Data Octamer Transcription Factor-1 Octamer Transcription Factor-2 Promoter Regions, Genetic/genetics RNA Polymerase II/genetics RNA Polymerase III/genetics RNA, Small Nuclear/genetics Regulatory Sequences, Nucleic Acid/genetics Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Transcription Factors/genetics,isolation & purification Transcription, Genetic
Chemicals
DNA Probes DNA-Binding Proteins HCFC1 protein, human Host Cell Factor C1 Octamer Transcription Factor-1 Octamer Transcription Factor-2 POU2F1 protein, human POU2F2 protein, human RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear SNAPC protein complex, human Transcription Factors RNA Polymerase II RNA Polymerase III
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Murphy S
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Yoon J B
Gerster T
Roeder R G
References (64)
64 references, click to expand
  1. Mammalian subtilisins: the long-sought dibasic processing endoproteases.
    Cell. 1991 Jul 12;66(1):1-3 PMID: 2070411
  2. POU-domain transcription factors: pou-er-ful developmental regulators.
    Genes Dev. 1991 Jun;5(6):897-907 PMID: 2044958
  3. Expression of a human 7S K RNA gene in vivo requires a novel pol III upstream element.
    EMBO J. 1990 Mar;9(3):711-8 PMID: 2138077
  4. Cooperative interactions between transcription factors Sp1 and OTF-1.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4732-6 PMID: 2191301
  5. Unraveling the complexities of transcription by RNA polymerase III.
    Trends Biochem Sci. 1990 Aug;15(8):300-4 PMID: 2204155
  6. Transcription factor Oct-2A contains functionally redundant activating domains and works selectively from a promoter but not from a remote enhancer position in non-lymphoid (HeLa) cells.
    EMBO J. 1990 May;9(5):1625-34 PMID: 2328728
  7. The cell type-specific octamer transcription factor OTF-2 has two domains required for the activation of transcription.
    EMBO J. 1990 May;9(5):1635-43 PMID: 2328729
  8. Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
    Mol Cell Biol. 1985 Jul;5(7):1560-70 PMID: 2410771
  9. Surprises in polymerase III transcription.
    Cell. 1988 Jan 29;52(2):153-4 PMID: 2449281
  10. Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen.
    J Biol Chem. 1989 Mar 25;264(9):5047-52 PMID: 2466842
  11. Epstein-Barr virus small RNA (EBER) genes: unique transcription units that combine RNA polymerase II and III promoter elements.
    Cell. 1989 Jun 2;57(5):825-34 PMID: 2541926
  12. Histone H1 subtype-specific consensus elements mediate cell cycle-regulated transcription in vitro.
    Genes Dev. 1989 Dec;3(12A):1982-90 PMID: 2620829
  13. A 7 bp mutation converts a human RNA polymerase II snRNA promoter into an RNA polymerase III promoter.
    Cell. 1989 Jul 14;58(1):55-67 PMID: 2752422
  14. Binding of the virion protein mediating alpha gene induction in herpes simplex virus 1-infected cells to its cis site requires cellular proteins.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7061-5 PMID: 2823252
  15. A herpesvirus trans-activating protein interacts with transcription factor OTF-1 and other cellular proteins.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6347-51 PMID: 2842768
  16. A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.
    Nature. 1988 Dec 8;336(6199):551-7 PMID: 2904654
  17. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
    Genes Dev. 1988 Dec;2(12A):1582-99 PMID: 2905684
  18. GAL4-VP16 is an unusually potent transcriptional activator.
    Nature. 1988 Oct 6;335(6190):563-4 PMID: 3047590
  19. Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro.
    Cell. 1987 Dec 4;51(5):783-93 PMID: 3119226
  20. Changing the RNA polymerase specificity of U snRNA gene promoters.
    Cell. 1988 Nov 4;55(3):435-42 PMID: 3180217
  21. Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.
    Mol Cell Biol. 1988 Oct;8(10):4028-40 PMID: 3185540
  22. Octamer-binding proteins from B or HeLa cells stimulate transcription of the immunoglobulin heavy-chain promoter in vitro.
    Genes Dev. 1988 Oct;2(10):1227-37 PMID: 3264542
  23. Upstream regulatory elements are necessary and sufficient for transcription of a U6 RNA gene by RNA polymerase III.
    EMBO J. 1988 Feb;7(2):503-12 PMID: 3366121
  24. Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif.
    Science. 1988 Jul 29;241(4865):577-80 PMID: 3399892
  25. The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.
    Cell. 1987 Oct 9;51(1):81-7 PMID: 3652210
  26. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  27. Structure of the nucleosome core particle at 7 A resolution.
    Nature. 1984 Oct 11-17;311(5986):532-7 PMID: 6482966
  28. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  29. TFIID is required for in vitro transcription of the human U6 gene by RNA polymerase III.
    EMBO J. 1991 Jul;10(7):1853-62 PMID: 2050122
  30. Activation of octamer-containing promoters by either octamer-binding transcription factor 1 (OTF-1) or OTF-2 and requirement of an additional B-cell-specific component for optimal transcription of immunoglobulin promoters.
    Mol Cell Biol. 1990 Dec;10(12):6204-15 PMID: 2123291
  31. Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4509-13 PMID: 2141169
  32. Use of T7 RNA polymerase to direct expression of cloned genes.
    Methods Enzymol. 1990;185:60-89 PMID: 2199796
  33. Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation.
    Cell. 1990 Feb 9;60(3):375-86 PMID: 2302733
  34. An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
    Nature. 1985 Jul 11-17;316(6024):163-7 PMID: 2409453
  35. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  36. Purified octamer binding transcription factors stimulate RNA polymerase III--mediated transcription of the 7SK RNA gene.
    Cell. 1989 Dec 22;59(6):1071-80 PMID: 2532066
  37. The Oct-1 homoeodomain directs formation of a multiprotein-DNA complex with the HSV transactivator VP16.
    Nature. 1989 Oct 19;341(6243):624-30 PMID: 2571937
  38. Common mechanisms of promoter recognition by RNA polymerases II and III.
    Trends Genet. 1989 Apr;5(4):122-6 PMID: 2658227
  39. Herpes simplex virus regulatory elements and the immunoglobulin octamer domain bind a common factor and are both targets for virion transactivation.
    Cell. 1988 Feb 12;52(3):435-45 PMID: 2830987
  40. A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells.
    Nature. 1988 Dec 8;336(6199):544-51 PMID: 2904653
  41. Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes.
    Nucleic Acids Res. 1987 Mar 25;15(6):2463-78 PMID: 3031599
  42. Identification of a novel lymphoid specific octamer binding protein (OTF-2B) by proteolytic clipping bandshift assay (PCBA).
    EMBO J. 1988 Dec 20;7(13):4221-9 PMID: 3072196
  43. Cell-cycle regulation of a human histone H2b gene is mediated by the H2b subtype-specific consensus element.
    Genes Dev. 1988 Jan;2(1):32-9 PMID: 3128460
  44. The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products.
    Genes Dev. 1988 Dec;2(12A):1513-6 PMID: 3215510
  45. Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used.
    Genes Dev. 1988 Feb;2(2):196-204 PMID: 3360322
  46. A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
    Cell. 1987 Oct 9;51(1):71-9 PMID: 3652209
  47. Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene.
    Cell. 1987 Dec 4;51(5):773-81 PMID: 3677172
  48. Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
    J Biol Chem. 1984 Jul 10;259(13):8345-52 PMID: 6203910
  49. TATA-binding protein is a classless factor.
    Cell. 1992 Mar 6;68(5):819-21 PMID: 1547484
  50. The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1.
    Cell. 1992 Mar 6;68(5):965-76 PMID: 1547496
  51. A role for the TATA-box-binding protein component of the transcription factor IID complex as a general RNA polymerase III transcription factor.
    Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1949-53 PMID: 1542692
  52. Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.
    Cell. 1992 Feb 21;68(4):755-67 PMID: 1739980
  53. Identification of transcription factors required for the expression of mammalian U6 genes in vitro.
    EMBO J. 1991 Sep;10(9):2595-603 PMID: 1868835
  54. Family of proteins that interact with TFIID and regulate promoter activity.
    Cell. 1991 Nov 1;67(3):557-67 PMID: 1934060
  55. Interactions of the Oct-1 POU subdomains with specific DNA sequences and with the HSV alpha-trans-activator protein.
    Genes Dev. 1990 Dec;4(12B):2383-96 PMID: 1980658
  56. RNA polymerase III transcription of genes that lack internal control regions.
    Biochim Biophys Acta. 1991 Jan 17;1088(1):1-9 PMID: 1989689
  57. A single base pair deletion from the inactive octamer-like motif of the 7S K distal sequence element brings full functionality in vivo.
    J Biol Chem. 1991 Dec 15;266(35):23872-7 PMID: 1721058
  58. Both Oct-1 and Oct-2A contain domains which can activate the ubiquitously expressed U2 snRNA genes.
    EMBO J. 1991 Aug;10(8):2291-6 PMID: 1829677
  59. The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.
    Genes Dev. 1991 Aug;5(8):1477-89 PMID: 1869050
  60. Differential phosphorylation of the transcription factor Oct1 during the cell cycle.
    Science. 1991 Aug 30;253(5023):1022-6 PMID: 1887216
  61. Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
    Nucleic Acids Res. 1991 Nov 25;19(22):6337-8 PMID: 1956801
  62. Participation of the TATA factor in transcription of the yeast U6 gene by RNA polymerase C.
    Science. 1991 Jan 25;251(4992):424-6 PMID: 1989075
  63. The different positioning of the proximal sequence element in the Xenopus RNA polymerase II and III snRNA promoters is a key determinant which confers RNA polymerase III specificity.
    Nucleic Acids Res. 1991 Feb 11;19(3):435-41 PMID: 2011518
  64. The human U1 snRNA promoter correctly initiates transcription in vitro and is activated by PSE1.
    Genes Dev. 1990 Dec;4(12A):2048-60 PMID: 2125285
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.7.3247-3261.1992
Published
1992-07-00
Pages
3247-61
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364539
Subset
IM
Grants
NCI NIH HHS · CA42567 · United States
NCRR NIH HHS · RR05869 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.7.3247-3261.1992 PMC Full Text View journal details
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