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PMID: 7885836 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoter control of translation in Xenopus oocytes.

Nucleic acids research ·Vol. 23 ·No. 3 ·1995-02-11 ·Pages 405-12

Gunkel N, Braddock M, Thorburn AM, Muckenthaler M, Kingsman AJ, Kingsman SM

Abstract

The HIV-1 promoter directs the high level production of transcripts in Xenopus oocytes. However, despite being exported to the cytoplasm, the transcripts are not translated [M. Braddock, A. M. Thorburn, A. Chambers, G. D. Elliott, G. J. Anderson, A. J. Kingsman and S. M. Kingsman (1990) Cell, 62, 1123-1133]. We have shown previously that this is a function of promoter sequences and is independent of the TAR RNA element that is normally located at the 5' end of all HIV mRNAs. We now show that a three nucleotide substitution at position -340, upstream of the RNA start site, reverses the translation inhibition. This site coincides with a sequence that can bind the haematopoietic transcription factor GATA. The inhibition of translation can also be reversed by treatment with inhibitors of casein kinase II or by injection into the nucleus of antibodies specific for the FRGY2 family of RNP proteins. We suggest that the -340 site influences the quality of the transcription complex such that transcripts are diverted to a nucleus-dependent translation inhibition pathway.

MeSH Terms
Animals Base Sequence Casein Kinase II Cell Nucleus/metabolism Cytoplasm/metabolism DNA/genetics,metabolism DNA-Binding Proteins/metabolism,pharmacology Erythroid-Specific DNA-Binding Factors HIV Long Terminal Repeat/genetics HIV-1/genetics Molecular Sequence Data Mutation/physiology Oocytes Promoter Regions, Genetic/genetics Protein Biosynthesis/drug effects,genetics Protein Serine-Threonine Kinases/antagonists & inhibitors Quercetin/pharmacology RNA, Messenger/biosynthesis,metabolism RNA-Binding Proteins/physiology Rutin/pharmacology Transcription Factors/metabolism,pharmacology,physiology Xenopus Proteins Xenopus laevis
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors RNA, Messenger RNA-Binding Proteins Transcription Factors Xenopus Proteins YBX2 protein, Xenopus Rutin DNA Quercetin Casein Kinase II Protein Serine-Threonine Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gunkel N
EMBL, Heidelberg, Germany.
Braddock M
Thorburn A M
Muckenthaler M
Kingsman A J
Kingsman S M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.3.405
Published
1995-02-11
Pages
405-12
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306690
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.3.405 PMC Full Text View journal details
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