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PMID: 7916585 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequencing of a tet(Q) gene isolated from Bacteroides fragilis 1126.

Antimicrobial agents and chemotherapy ·Vol. 37 ·No. 9 ·1993-09-00 ·Pages 2037-41

Lépine G, Lacroix JM, Walker CB, Progulske-Fox A

Abstract

Recently, Tet Q, a tetracycline resistance determinant that confers resistance by a ribosome protection mechanism, was described and added to the two previously described classes, Tet M and Tet O. The first representative of this class, tetA(Q)1, was isolated from Bacteroides thetaiotaomicron DOT. We report the sequencing of a gene isolated from B. fragilis 1126 which also confers tetracycline resistance. Because of its high degree of identity (97%) with the tetA(Q)1 gene, we defined it as tetA(Q)2. MIC studies revealed that tetA(Q)2 provides a low level of resistance to tetracycline when cloned into Escherichia coli. The extensive homology between tetA(Q)1 and tetA(Q)2 supports the idea of a recent horizontal transfer of tet(Q) genes among Bacteroides spp.

Related Genes
tetA(Q)1 tetA(Q)2
MeSH Terms
Amino Acid Sequence Bacteroides fragilis/genetics Base Sequence Cloning, Molecular DNA, Recombinant/isolation & purification Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Plasmids Protein Biosynthesis Sequence Homology, Nucleic Acid Tetracycline Resistance/genetics Transformation, Bacterial
Chemicals
DNA, Recombinant
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lépine G
Periodontal Disease Research Center, College of Dentistry, University of Florida, Gainesville 32610.
Lacroix J M
Walker C B
Progulske-Fox A
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.37.9.2037
Published
1993-09-00
Pages
2037-41
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC188119
Subset
IM
Grants
NIDCR NIH HHS · DE06070 · United States
NIDCR NIH HHS · DE07496 · United States
Databases
GENBANK
L05008, L05009, L05010, L05011, L05012, L05013, L05014, L08748, M97169, Z21523
External Links
PubMed source DOI: 10.1128/AAC.37.9.2037 PMC Full Text View journal details
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