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PMID: 8036168 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and functional characterization of LCR-F1: a bZIP transcription factor that activates erythroid-specific, human globin gene expression.

Nucleic acids research ·Vol. 22 ·No. 12 ·1994-06-25 ·Pages 2383-91

Caterina JJ, Donze D, Sun CW, Ciavatta DJ, Townes TM

Abstract

DNase I hypersensitive site 2 (HS 2) of the human beta-globin Locus Control Region (LCR) directs high level expression of the beta-globin gene located 50 kilobases downstream. Experiments in cultured cells and in transgenic mice demonstrate that duplicated AP1-like sites in HS 2 are required for this powerful enhancer activity. A cDNA clone encoding a basic, leucine-zipper protein that binds to these sites was isolated and designated Locus Control Region-Factor 1 (LCR-F1). This protein is a member of a new family of regulatory factors that contain a 63 amino acid 'CNC domain' overlapping the basic region. This domain is approximately 70% identical in the Drosophila Cap N Collar (CNC) protein, NF-E2 and LCR-F1. LCR-F1 transactivates an HS 2/gamma-globin reporter gene over 170-fold in transient transfection experiments specifically in erythroid cells. These results suggest that LCR-F1 may be a critical factor involved in LCR-mediated, human globin gene expression.

MeSH Terms
Animals Base Sequence Binding Sites Cloning, Molecular DNA DNA-Binding Proteins/genetics,metabolism Erythrocytes/metabolism Erythroid-Specific DNA-Binding Factors Gene Expression Regulation Globins/biosynthesis,genetics HeLa Cells Humans Leukemia, Erythroblastic, Acute Mice Mice, Transgenic Molecular Sequence Data Mutation NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit NF-E2-Related Factor 1 Recombinant Fusion Proteins/genetics,metabolism Sequence Homology, Amino Acid Transcription Factors/genetics,metabolism Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit NF-E2-Related Factor 1 NFE2 protein, human NFE2L1 protein, human Nfe2 protein, mouse Recombinant Fusion Proteins Transcription Factors Globins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Caterina J J
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham 35294.
Donze D
Sun C W
Ciavatta D J
Townes T M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.12.2383
Published
1994-06-25
Pages
2383-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC523699
Subset
IM
Grants
NHLBI NIH HHS · HL35559 · United States
NHLBI NIH HHS · HL43508 · United States
NCI NIH HHS · NCI CA13148 · United States
Databases
GENBANK
U08853
External Links
PubMed source DOI: 10.1093/nar/22.12.2383 PMC Full Text View journal details
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