Home LiteratureArticle Details
PMID: 8083178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Iron-responsive genetic regulation in Campylobacter jejuni: cloning and characterization of a fur homolog.

Journal of bacteriology ·Vol. 176 ·No. 18 ·1994-09-00 ·Pages 5852-6

Wooldridge KG, Williams PH, Ketley JM

Abstract

The Fur protein of Escherichia coli represses transcription from Fur-responsive genes in an iron-dependent manner. We have demonstrated a Fur-like iron-responsive genetic regulatory activity operating in Campylobacter jejuni by using a chloramphenicol acetyl transferase reporter gene separated from its promoter by a synthetic Fur-responsive operator. A fur-like gene has been cloned from C. jejuni by partial functional complementation of an E. coli fur mutation. Sequence analysis has shown that, at the amino acid level, the C. jejuni Fur protein is 35% identical with its E. coli counterpart.

Related Genes
fur
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Campylobacter jejuni/genetics Chloramphenicol O-Acetyltransferase/genetics Cloning, Molecular Gene Expression Regulation, Bacterial/drug effects Genes, Bacterial/genetics Genes, Reporter/genetics Genetic Complementation Test Iron/pharmacology Molecular Sequence Data Operator Regions, Genetic/genetics Repressor Proteins/genetics Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Repressor Proteins ferric uptake regulating proteins, bacterial Iron Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wooldridge K G
Department of Genetics, University of Leicester, England.
Williams P H
Ketley J M
References (37)
37 references, click to expand
  1. Iron regulation of Shiga-like toxin expression in Escherichia coli is mediated by the fur locus.
    J Bacteriol. 1987 Oct;169(10):4759-64 PMID: 3308853
  2. Investigations on the role of flagella in the colonization of infant mice with Campylobacter jejuni and attachment of Campylobacter jejuni to human epithelial cell lines.
    J Hyg (Lond). 1985 Oct;95(2):217-27 PMID: 4067286
  3. The Campylobacter sigma 54 flaB flagellin promoter is subject to environmental regulation.
    J Bacteriol. 1993 Jul;175(14):4448-55 PMID: 8331072
  4. Metalloregulation in Bacillus subtilis: isolation and characterization of two genes differentially repressed by metal ions.
    J Bacteriol. 1993 Sep;175(17):5428-37 PMID: 8396117
  5. Influence of iron on growth, morphology, outer membrane protein composition, and synthesis of siderophores in Campylobacter jejuni.
    Infect Immun. 1986 Oct;54(1):126-32 PMID: 2944843
  6. Identification and cloning of a fur homolog from Neisseria gonorrhoeae.
    Infect Immun. 1993 Nov;61(11):4599-606 PMID: 8406856
  7. Antigenic variation of Campylobacter flagella.
    J Bacteriol. 1987 Nov;169(11):5066-71 PMID: 2444577
  8. Confirmation of the Fur operator site by insertion of a synthetic oligonucleotide into an operon fusion plasmid.
    J Bacteriol. 1988 Feb;170(2):1015-7 PMID: 2828307
  9. Selection procedure for deregulated iron transport mutants (fur) in Escherichia coli K 12: fur not only affects iron metabolism.
    Mol Gen Genet. 1987 Nov;210(1):135-9 PMID: 3323834
  10. Molecular mechanism of regulation of siderophore-mediated iron assimilation.
    Microbiol Rev. 1987 Dec;51(4):509-18 PMID: 2963952
  11. Iron regulation of the cloned diphtheria toxin promoter in Escherichia coli.
    Infect Immun. 1988 Sep;56(9):2430-6 PMID: 3044999
  12. Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5968-72 PMID: 2116013
  13. Chloramphenicol resistance in Campylobacter coli: nucleotide sequence, expression, and cloning vector construction.
    Gene. 1990 Sep 28;94(1):23-8 PMID: 2227449
  14. Identification and cloning of a fur regulatory gene in Yersinia pestis.
    J Bacteriol. 1991 Jan;173(2):417-25 PMID: 1898928
  15. Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion.
    EMBO J. 1991 Aug;10(8):2055-61 PMID: 2065653
  16. Structural dynamics and functional domains of the fur protein.
    Biochemistry. 1991 Aug 20;30(33):8201-10 PMID: 1868094
  17. Lysyl-tRNA synthetase gene of Campylobacter jejuni.
    J Bacteriol. 1992 Feb;174(3):695-701 PMID: 1732205
  18. Cloning, sequencing, and transcriptional regulation of the Vibrio cholerae fur gene.
    J Bacteriol. 1992 Mar;174(6):1897-903 PMID: 1372314
  19. Iron acquisition and hemolysin production by Campylobacter jejuni.
    Infect Immun. 1992 Sep;60(9):3872-7 PMID: 1500194
  20. Cloning and genetic analysis of the Vibrio vulnificus fur gene and construction of a fur mutant by in vivo marker exchange.
    J Bacteriol. 1993 Feb;175(3):706-15 PMID: 7678593
  21. Neisseria meningitidis produces iron-regulated proteins related to the RTX family of exoproteins.
    J Bacteriol. 1993 Feb;175(3):811-8 PMID: 8423153
  22. Coordinate regulation of siderophore and exotoxin A production: molecular cloning and sequencing of the Pseudomonas aeruginosa fur gene.
    J Bacteriol. 1993 May;175(9):2589-98 PMID: 8478325
  23. Mechanisms for negative regulation by iron of the fatA outer membrane protein gene expression in Vibrio anguillarum 775.
    J Biol Chem. 1993 May 15;268(14):10433-9 PMID: 7683679
  24. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  25. Effect of iron on yields of exotoxin A in cultures of Pseudomonas aeruginosa PA-103.
    Infect Immun. 1978 Mar;19(3):785-91 PMID: 417030
  26. Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures.
    J Bacteriol. 1979 Apr;138(1):193-200 PMID: 108250
  27. Campylobacter urinary infection.
    Lancet. 1979 May 19;1(8125):1091-2 PMID: 86820
  28. Campylobacter cholecystitis.
    Lancet. 1979 Jun 16;1(8129):1302 PMID: 87770
  29. Campylobacter enteritis.
    Clin Gastroenterol. 1979 Sep;8(3):737-65 PMID: 387302
  30. Enteritis and septicaemia due to Campylobacter jejuni.
    Acta Clin Belg. 1979;34(6):365-9 PMID: 554396
  31. Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant.
    Mol Gen Genet. 1981;182(2):288-92 PMID: 7026976
  32. The significance of iron in infection.
    Rev Infect Dis. 1981 Nov-Dec;3(6):1127-38 PMID: 7043704
  33. Epidemiology of Campylobacter jejuni infections.
    Epidemiol Rev. 1983;5:157-76 PMID: 6357819
  34. The relationship of plasmid-mediated iron transport and bacterial virulence.
    Annu Rev Microbiol. 1984;38:69-89 PMID: 6093687
  35. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  36. Reversible expression of flagella in Campylobacter jejuni.
    Infect Immun. 1985 Dec;50(3):941-3 PMID: 4066041
  37. Nosocomial outbreak of Campylobacter jejuni meningitis in newborn infants.
    Lancet. 1986 Jul 19;2(8499):146-9 PMID: 2873408
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.18.5852-5856.1994
Published
1994-09-00
Pages
5852-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196792
Subset
IM
Grants
Wellcome Trust · United Kingdom
Databases
GENBANK
X78965
External Links
PubMed source DOI: 10.1128/jb.176.18.5852-5856.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2