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PMID: 8096814 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A mitochondrial import factor purified from rat liver cytosol is an ATP-dependent conformational modulator for precursor proteins.

The EMBO journal ·Vol. 12 ·No. 4 ·1993-04-00 ·Pages 1579-86

Hachiya N, Alam R, Sakasegawa Y, Sakaguchi M, Mihara K, Omura T

Abstract

Rat liver cytosol contained an activity that stimulated the import of wheat germ lysate-synthesized precursor proteins into mitochondria. The activity was purified 10,000-fold from the cytosol as a homogeneous heterodimeric protein. This protein (termed mitochondrial import stimulation factor or MSF) stimulated the binding and import of mitochondrial precursor proteins. MSF was also found to recognize the presequence portion of mitochondrial precursors and catalyze the depolymerization and unfolding of in vitro synthesized mitochondrial precursor proteins in an ATP-dependent manner; in this connection, MSF exhibited ATPase activity depending on the important-incompetent mitochondrial precursor protein. The mitochondrial binding and import-stimulating activities were strongly inhibited by the pretreatment of MSF with NEM, whereas the ATP-dependent depolymerization activity was insensitive to the NEM treatment, suggesting that the process subsequent to the unfolding was inhibited with the NEM treatment. We conclude that MSF is a multifunctional cytoplasmic chaperone specific for mitochondrial protein import.

MeSH Terms
Adenosine Triphosphatases/isolation & purification,metabolism Adenosine Triphosphate/metabolism Animals Biological Transport Chaperonins Cytosol/metabolism Electron Transport Complex IV/metabolism Ethylmaleimide/pharmacology Liver/metabolism Mitochondria, Liver/metabolism Polymers Protein Precursors/metabolism Proteins/isolation & purification,metabolism Rats Recombinant Proteins/metabolism
Chemicals
Polymers Protein Precursors Proteins Recombinant Proteins Adenosine Triphosphate Electron Transport Complex IV Adenosine Triphosphatases Chaperonins Ethylmaleimide
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hachiya N
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
Alam R
Sakasegawa Y
Sakaguchi M
Mihara K
Omura T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-04-00
Pages
1579-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413371
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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