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PMID: 810087 Published · ppublish English Journal Article

Degradation of 3-hydroxybenzoate by bacteria of the genus Bacillus.

Applied microbiology ·Vol. 30 ·No. 3 ·1975-09-00 ·Pages 439-44

Crawford RL

Abstract

The pathway whereby certain bacterial strains of the genus Bacillus degrade m-hydroxybenzoate is delineated. Of 12 strains examined, nine were tentatively classified as representatives of the species Bacillus brevis, two of Bacillus sphaericus and one of Bacillus megaterium. All strains degraded m-hydroxybenzoate via the same pathway. m-Hydroxybenzoate was hydroxylated to 2,5-dihydroxybenzoate (gentisate), which was oxidized by a gentisate 1,2-deoxygenase yielding maleylpyruvate. Maleylpyruvate was hydrolyzed without prior cis, cis to cis, trans isomerization yielding pyruvate and maleic acid. Numerous soils were examined by plate-count procedures and found to contain 10(4) to 10(6) aerobic sporeformers able to grow on m-hydroxybenzoate per g of dry soil.

MeSH Terms
Bacillus/enzymology,metabolism Bacillus megaterium/enzymology,metabolism Biodegradation, Environmental Cell-Free System Fumarate Hydratase/metabolism Gentisates/metabolism Hydrolases/metabolism Hydroxybenzoates/metabolism Isomerases/metabolism Maleates/biosynthesis Oxygenases/metabolism Pyruvates/biosynthesis Soil Microbiology
Chemicals
Gentisates Hydroxybenzoates Maleates Pyruvates Oxygenases Hydrolases Fumarate Hydratase Isomerases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Crawford R L
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Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.30.3.439-444.1975
Published
1975-09-00
Pages
439-44
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC187200
Subset
IM
External Links
PubMed source DOI: 10.1128/am.30.3.439-444.1975 PMC Full Text
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