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PMID: 8107211 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Subcellular distribution of human immunodeficiency virus type 1 Rev and colocalization of Rev with RNA splicing factors in a speckled pattern in the nucleoplasm.

Journal of virology ·Vol. 68 ·No. 3 ·1994-03-00 ·Pages 1475-85

Kalland KH, Szilvay AM, Langhoff E, Haukenes G

Abstract

The human immunodeficiency virus type 1 (HIV-1) Rev (regulator of virion protein expression) protein exemplifies a new type of posttranscriptional regulation. One main function of Rev is to increase the cytoplasmic expression of unspliced and incompletely spliced retroviral mRNAs from which viral structural proteins are made. In that way, Rev is essential in order to complete the retroviral life cycle. The biology of Rev in the host cell has remained elusive. In this study, a complex distribution of Rev in single cells was found. Rev was found in the cytoplasm, in a perinuclear zone, in the nucleoplasm, and in the nucleoli. In the nucleoplasm, Rev colocalized in a speckled pattern with host cell factors known to assemble on nascent transcripts. Those factors are involved in the processing of heterogeneous RNA to spliced mRNA in the nucleoplasm of all cells. The distribution of Rev was dependent only on Rev and host cell interactions, since neither the Rev target RNA nor other HIV proteins were expressed in the cells. Rev was found in the same subcellular compartments of cells treated for extended periods with cycloheximide, an inhibitor of protein synthesis. This finding implies that Rev shuttles continuously between cytoplasmic and nucleoplasmic compartments. The results suggest a potential role for Rev both in the RNA-splicing process and in the nucleocytoplasmic transport of Rev-dependent HIV mRNA.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/biosynthesis Antibody Specificity Autoantibodies Biological Transport Cell Compartmentation Cell Nucleus/chemistry,ultrastructure Cycloheximide/pharmacology Fluorescent Antibody Technique Gene Products, rev/immunology,isolation & purification HIV Antibodies/biosynthesis HIV-1/chemistry,immunology HeLa Cells Humans Interphase/physiology Molecular Sequence Data Protein Biosynthesis/drug effects RNA Splicing RNA, Small Nuclear/isolation & purification Ribonucleoproteins, Small Nuclear/isolation & purification rev Gene Products, Human Immunodeficiency Virus
Chemicals
Antibodies, Monoclonal Autoantibodies Gene Products, rev HIV Antibodies RNA, Small Nuclear Ribonucleoproteins, Small Nuclear rev Gene Products, Human Immunodeficiency Virus Cycloheximide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kalland K H
Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.
Szilvay A M
Langhoff E
Haukenes G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1994-03-00
Pages
1475-85
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236603
Subset
IM
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