Home LiteratureArticle Details
PMID: 8164671 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The Glc7 type 1 protein phosphatase of Saccharomyces cerevisiae is required for cell cycle progression in G2/M.

Molecular and cellular biology ·Vol. 14 ·No. 5 ·1994-05-00 ·Pages 3158-65

Hisamoto N, Sugimoto K, Matsumoto K

Abstract

We isolated a mutant carrying a conditional mutation in the GLC7 gene, encoding the catalytic subunit of a type 1 protein phosphatase, by selection of suppressors that restored the growth defect of cdc24 mutants at high temperature and simultaneously conferred cold-sensitive growth. This cold sensitivity for growth is caused by a single mutation (glc7Y-170) at position 170 of the Glc7 protein, resulting in replacement of cysteine with tyrosine. Genetic analysis suggested that the glc7Y-170 allele is associated with a recessive negative phenotype, reducing the activity of Glc7 in the cell. The glc7Y-170 mutant missegregated chromosome III at the permissive temperature, arrested growth as large-budded cells at the restrictive temperature, exhibited a significant increase in the number of nuclei at or in the neck, and had a short spindle. Furthermore, the glc7Y-170 mutant exhibited a high level of CDC28-dependent protein kinase activity when incubated at the restrictive temperature. These findings suggest that the glc7Y-170 mutation is defective in the G2/M phase of the cell cycle. Thus, type 1 protein phosphatase in Saccharomyces cerevisiae is essential for the G2/M transition.

Related Genes
GLC7 STF1
MeSH Terms
Amino Acid Sequence Base Sequence CDC28 Protein Kinase, S cerevisiae/metabolism Cell Cycle Chromosome Mapping Chromosomes, Fungal Cloning, Molecular Fungal Proteins/biosynthesis,genetics,metabolism G2 Phase Genes, Fungal Genetic Complementation Test Genotype Glycogen/metabolism Mitosis Molecular Sequence Data Mutagenesis Phenotype Phosphoprotein Phosphatases/biosynthesis,genetics,metabolism Plasmids Protein Kinases/metabolism Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics,growth & development Sequence Homology, Amino Acid
Chemicals
Fungal Proteins Glycogen Protein Kinases CDC28 Protein Kinase, S cerevisiae Phosphoprotein Phosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hisamoto N
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.
Sugimoto K
Matsumoto K
References (44)
44 references, click to expand
  1. Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: homology with protein phosphatase 2A.
    FEBS Lett. 1987 Nov 2;223(2):340-6 PMID: 2822491
  2. Improved method for high efficiency transformation of intact yeast cells.
    Nucleic Acids Res. 1992 Mar 25;20(6):1425 PMID: 1561104
  3. Protein phosphatase type 1 in mammalian cell mitosis: chromosomal localization and involvement in mitotic exit.
    J Cell Biol. 1992 Mar;116(6):1421-30 PMID: 1311712
  4. The structure and regulation of protein phosphatases.
    Annu Rev Biochem. 1989;58:453-508 PMID: 2549856
  5. Identification of essential components of the S. cerevisiae kinetochore.
    Cell. 1993 May 21;73(4):761-74 PMID: 8500169
  6. Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
    Nature. 1987 May 7-13;327(6117):31-5 PMID: 3553962
  7. Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation.
    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9006-10 PMID: 1717994
  8. Mitotic regulation of protein phosphatases by the fission yeast sds22 protein.
    Curr Biol. 1993 Jan;3(1):13-26 PMID: 15335873
  9. The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M.
    EMBO J. 1990 Dec;9(13):4347-58 PMID: 2265610
  10. The yeast GLC7 gene required for glycogen accumulation encodes a type 1 protein phosphatase.
    J Biol Chem. 1991 Dec 15;266(35):23796-801 PMID: 1660885
  11. One of the protein phosphatase 1 isoenzymes in Drosophila is essential for mitosis.
    Cell. 1990 Oct 5;63(1):33-46 PMID: 2170019
  12. Truncated protein phosphatase GLC7 restores translational activation of GCN4 expression in yeast mutants defective for the eIF-2 alpha kinase GCN2.
    Mol Cell Biol. 1992 Dec;12(12):5700-10 PMID: 1333044
  13. Substrates for p34cdc2: in vivo veritas?
    Cell. 1990 May 18;61(4):549-51 PMID: 2188729
  14. Cold-sensitive and caffeine-supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis.
    EMBO J. 1988 May;7(5):1465-73 PMID: 3409871
  15. The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
    Cell. 1989 Jun 16;57(6):997-1007 PMID: 2544298
  16. A brain serine/threonine protein kinase activated by Cdc42 and Rac1.
    Nature. 1994 Jan 6;367(6458):40-6 PMID: 8107774
  17. Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
    Cell. 1989 Mar 10;56(5):829-38 PMID: 2538242
  18. Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1 alpha. High conservation between mammalian and insect sequences.
    Eur J Biochem. 1989 Aug 15;183(3):603-10 PMID: 2550221
  19. S. pombe gene sds22+ essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1.
    Cell. 1991 Jan 11;64(1):149-57 PMID: 1846086
  20. Heritable glycogen-storage deficiency in yeast and its induction by ultra-violet light.
    J Gen Microbiol. 1968 Apr;51(1):49-56 PMID: 5653230
  21. Driving the cell cycle: M phase kinase, its partners, and substrates.
    Cell. 1990 Jun 1;61(5):743-52 PMID: 2160858
  22. The bimG gene of Aspergillus nidulans, required for completion of anaphase, encodes a homolog of mammalian phosphoprotein phosphatase 1.
    Cell. 1989 Jun 16;57(6):987-96 PMID: 2544297
  23. Mammalian growth-associated H1 histone kinase: a homolog of cdc2+/CDC28 protein kinases controlling mitotic entry in yeast and frog cells.
    Mol Cell Biol. 1989 Sep;9(9):3860-8 PMID: 2550805
  24. Protein serine/threonine kinases.
    Annu Rev Biochem. 1987;56:567-613 PMID: 2956925
  25. GAC1 may encode a regulatory subunit for protein phosphatase type 1 in Saccharomyces cerevisiae.
    EMBO J. 1992 Jan;11(1):87-96 PMID: 1310938
  26. Mitotic control by metaphase-promoting factor and cdc proteins.
    J Cell Sci. 1989 Feb;92 ( Pt 2):131-5 PMID: 2674165
  27. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  28. Mutations in yeast calmodulin cause defects in spindle pole body functions and nuclear integrity.
    J Cell Biol. 1992 Dec;119(6):1625-39 PMID: 1469052
  29. Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies.
    J Cell Sci. 1989 Jul;93 ( Pt 3):491-500 PMID: 2606940
  30. Altered fidelity of mitotic chromosome transmission in cell cycle mutants of S. cerevisiae.
    Genetics. 1985 Jul;110(3):381-95 PMID: 3894160
  31. Control of the yeast cell cycle is associated with assembly/disassembly of the Cdc28 protein kinase complex.
    Cell. 1988 Sep 23;54(7):1061-72 PMID: 3046752
  32. The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe.
    J Cell Sci. 1988 Mar;89 ( Pt 3):343-57 PMID: 3198697
  33. Genetic interaction between the Ras-cAMP pathway and the Dis2s1/Glc7 protein phosphatase in Saccharomyces cerevisiae.
    Mol Gen Genet. 1994 Feb;242(3):257-62 PMID: 8107672
  34. Purification and characterization of glycogen synthase from a glycogen-deficient strain of Saccharomyces cerevisiae.
    J Biol Chem. 1990 Aug 15;265(23):13871-7 PMID: 2116410
  35. Regulation of p34cdc2 protein kinase during mitosis.
    Cell. 1989 Jul 28;58(2):361-72 PMID: 2665944
  36. Use of T7 RNA polymerase to direct expression of cloned genes.
    Methods Enzymol. 1990;185:60-89 PMID: 2199796
  37. Specific enzymatic dephosphorylation of the retinoblastoma protein.
    Mol Cell Biol. 1993 Jan;13(1):367-72 PMID: 8380224
  38. Roles of the CDC24 gene product in cellular morphogenesis during the Saccharomyces cerevisiae cell cycle.
    J Cell Biol. 1981 Jun;89(3):395-405 PMID: 7019215
  39. p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.
    EMBO J. 1987 Nov;6(11):3507-14 PMID: 3322810
  40. The mutant type 1 protein phosphatase encoded by glc7-1 from Saccharomyces cerevisiae fails to interact productively with the GAC1-encoded regulatory subunit.
    Mol Cell Biol. 1994 Feb;14(2):896-905 PMID: 8289829
  41. Involvement of a type 1 protein phosphatase encoded by bws1+ in fission yeast mitotic control.
    Cell. 1989 Jun 16;57(6):1009-16 PMID: 2544292
  42. Signal integration at the level of protein kinases, protein phosphatases and their substrates.
    Trends Biochem Sci. 1992 Oct;17(10):408-13 PMID: 1333658
  43. Mutational analysis of CDC42Sc, a Saccharomyces cerevisiae gene that encodes a putative GTP-binding protein involved in the control of cell polarity.
    Mol Cell Biol. 1991 Jul;11(7):3537-44 PMID: 1904541
  44. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components.
    EMBO J. 1992 Dec;11(13):4815-24 PMID: 1464311
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.5.3158-3165.1994
Published
1994-05-00
Pages
3158-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358683
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.5.3158-3165.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2