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PMID: 8290347 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription of a silkworm tRNA(cAla) gene is directed by two AT-rich upstream sequence elements.

Nucleic acids research ·Vol. 21 ·No. 25 ·1993-12-25 ·Pages 5875-81

Palida FA, Hale C, Sprague KU

Abstract

A region within 35 nucleotides upstream of the transcription initiation site of a variety of silkworm Class III templates is absolutely required for transcription in vitro. To determine whether the activity of this region can be attributed to a particular sequence element, we systematically replaced 4-5 bp segments of the region upstream of a silkworm tRNA(cAla) gene. We show that replacement of either of two AT-rich blocks markedly impairs promoter function, whereas replacement of other sequences has little or no effect. Additional mutants were constructed to test whether base composition or sequence is important for function of the AT blocks. We find that some sequences are more effective than others, but that various AT-rich sequences can direct transcription at a high level. Possible mechanisms by which such elements could act are discussed.

MeSH Terms
Animals Base Composition Base Sequence Bombyx Cloning, Molecular DNA Humans Molecular Sequence Data Mutagenesis, Site-Directed Promoter Regions, Genetic RNA, Transfer, Ala/genetics Transcription, Genetic
Chemicals
RNA, Transfer, Ala DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Palida F A
Institute of Molecular Biology, University of Oregon, Eugene 97403.
Hale C
Sprague K U
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.25.5875
Published
1993-12-25
Pages
5875-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310468
Subset
IM
Grants
NIGMS NIH HHS · GM25388 · United States
External Links
PubMed source DOI: 10.1093/nar/21.25.5875 PMC Full Text View journal details
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