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PMID: 8349778 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates.

Journal of clinical microbiology ·Vol. 31 ·No. 7 ·1993-07-00 ·Pages 1927-31

Woods CR, Versalovic J, Koeuth T, Lupski JR

Abstract

Repetitive element sequence-based polymerase chain reaction (rep-PCR) enables the generation of DNA fingerprints which discriminate bacterial species and strains. We describe the application of whole-cell methods which allow specimens from broth cultures or colonies from agar plates to be utilized directly in the PCR mixture. The rep-PCR-generated DNA fingerprints obtained with whole-cell samples match results obtained with genomic DNA templates. Examples with different gram-negative bacteria (e.g., Citrobacter diversus, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive bacteria (e.g., Staphylococcus aureus and Streptococcus pneumoniae) are demonstrated. Rapid specimen preparation methods enable rep-PCR-based fingerprinting to be completed in several hours and, therefore, allows the timely analysis of epidemiological relationships.

MeSH Terms
Bacteriological Techniques DNA Fingerprinting DNA, Bacterial/genetics Evaluation Studies as Topic Gram-Negative Bacteria/classification,genetics,isolation & purification Gram-Positive Bacteria/classification,genetics,isolation & purification Humans Polymerase Chain Reaction/methods Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Woods C R
Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030.
Versalovic J
Koeuth T
Lupski J R
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.31.7.1927-1931.1993
Published
1993-07-00
Pages
1927-31
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265663
Subset
IM
Grants
PHS HHS · 1F31C-M14601-01 SRC-7 · United States
External Links
PubMed source DOI: 10.1128/jcm.31.7.1927-1931.1993 PMC Full Text View journal details
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