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PMID: 1637156 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria.

Applied and environmental microbiology ·Vol. 58 ·No. 7 ·1992-07-00 ·Pages 2180-7

de Bruijn FJ

Abstract

The distribution of dispersed repetitive DNA (repetitive extragenic palindromic [REP] and enterobacterial repetitive intergenic consensus [ERIC]) sequences in the genomes of a number of gram-negative soil bacteria was examined by using conserved primers corresponding to REP and ERIC sequences and the polymerase chain reaction (PCR). The patterns of the resulting PCR products were analyzed on agarose gels and found to be highly specific for each strain. The REP and ERIC PCR patterns of a series of Rhizobium meliloti isolates, previously ordered in a phylogenetic tree based on allelic variations at 14 enzyme loci (B. D. Eardly, L. A. Materon, N. H. Smith, D. A. Johnson, M. D. Rumbaugh, and R. K. Selander, Appl. Environ. Microbiol. 56:187-194), were determined. Isolates which had been postulated to be closely related by multilocus enzyme electrophoresis also revealed similar REP and ERIC PCR patterns, suggesting that the REP and ERIC PCR method is useful for the identification and classification of bacterial strains.

MeSH Terms
Base Sequence DNA Fingerprinting DNA, Bacterial/genetics Escherichia coli/genetics Genome, Bacterial Molecular Sequence Data Polymerase Chain Reaction Pseudomonas/genetics Repetitive Sequences, Nucleic Acid Rhizobium/genetics Sinorhizobium meliloti/classification,genetics Soil Microbiology Species Specificity
Chemicals
DNA, Bacterial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
de Bruijn F J
MSU-DOE Plant Research Laboratory, Department of Microbiology, E. Lansing, Michigan.
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.58.7.2180-2187.1992
Published
1992-07-00
Pages
2180-7
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC195753
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.58.7.2180-2187.1992 PMC Full Text View journal details
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