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PMID: 8408204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protein transport to the dendritic plasma membrane of cultured neurons is regulated by rab8p.

The Journal of cell biology ·Vol. 123 ·No. 1 ·1993-10-00 ·Pages 47-55

Huber LA, de Hoop MJ, Dupree P, Zerial M, Simons K, Dotti C

Abstract

In the companion paper (Huber, L. A., S. W. Pimplikar, R. G. Parton, H. Virta, M. Zerial, and K. Simons. J. Cell Biol. 123:35-45) we reported that the small GTPase rab8p is involved in transport from the TGN to the basolateral plasma membrane in epithelia. In the present work we investigated the localization and function of rab8p in polarized hippocampal neurons. By immunofluorescence microscopy we found that rab8p localized preferentially in the somatodendritic domain, and was excluded from the axon. Double-labeling immunofluorescence showed that some of the rab8p co-localized in the dendrites with the Semliki Forest Virus glycoprotein E2 (SFV-E2). An antisense oligonucleotide approach was used to investigate the role of rab8p in dendritic transport of newly synthesized viral glycoproteins. Antisense oligonucleotides corresponding to the initiation region of the rab8 coding sequence were added to the cultured neurons for four days. This treatment resulted in a significant decrease in cellular levels of rab8p and transport of SFV-E2 from the cell body to the dendrites was significantly reduced. However, no effect was observed on axonal transport of influenza HA. From these results we conclude that rab8p is involved in transport of proteins to the dendritic surface in neurons.

MeSH Terms
Animals Base Sequence Biological Transport/drug effects Cell Compartmentation Cell Membrane/metabolism Cells, Cultured DNA, Antisense/pharmacology Dendrites/metabolism,ultrastructure Fluorescent Antibody Technique GTP-Binding Proteins/metabolism Gene Expression Regulation/drug effects Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/metabolism Hippocampus/cytology Molecular Sequence Data Neurons/cytology,metabolism Oligonucleotides, Antisense/pharmacology Rats Viral Envelope Proteins/metabolism Viral Proteins/metabolism rab GTP-Binding Proteins
Chemicals
DNA, Antisense Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral Oligonucleotides, Antisense Viral Envelope Proteins Viral Proteins GTP-Binding Proteins Rab8b protein, rat rab GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Huber L A
Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
de Hoop M J
Dupree P
Zerial M
Simons K
Dotti C
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.123.1.47
Published
1993-10-00
Pages
47-55
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119825
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.123.1.47 PMC Full Text View journal details
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