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PMID: 8413244 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

PU.1 is a component of a multiprotein complex which binds an essential site in the murine immunoglobulin lambda 2-4 enhancer.

Molecular and cellular biology ·Vol. 13 ·No. 10 ·1993-10-00 ·Pages 6452-61

Eisenbeis CF, Singh H, Storb U

Abstract

B-cell-specific enhancers have been identified in the immunoglobulin lambda locus 3' of each constant-region cluster. These enhancers contain two distinct domains, lambda A and lambda B, which are essential for enhancer function. lambda B contains a near-consensus binding site for the Ets family of transcription factors. In this study, we have identified a B-cell-specific protein complex which binds the lambda B motif of the lambda 2-4 enhancer in vitro and appears necessary for the activity of the enhancer in vivo, since mutations in lambda B which prevent this interaction also eliminate enhancer function. This complex contains PU.1, a member of the Ets family, and a transcriptional activator whose expression is restricted to cells of the hematopoietic system with the exception of T lymphocytes. In addition, it contains a factor which binds specifically to a region adjacent to the PU.1 binding site. This factor cannot bind lambda B autonomously but appears to require interaction with the PU.1 protein to stabilize its association with the DNA. This complex may be identical or related to the PU.1/NF-EM5 complex which interacts with a homologous DNA element in the immunoglobulin kappa 3' enhancer.

MeSH Terms
Animals Base Sequence Binding, Competitive DNA DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Immunoglobulin lambda-Chains/genetics Macromolecular Substances Methylation Mice Molecular Sequence Data Multiprotein Complexes Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins c-ets Retroviridae Proteins, Oncogenic Transcription Factors
Chemicals
DNA-Binding Proteins Immunoglobulin lambda-Chains Macromolecular Substances Multiprotein Complexes Proto-Oncogene Proteins Proto-Oncogene Proteins c-ets Retroviridae Proteins, Oncogenic Transcription Factors v-Spi-1 protein, Friend spleen focus-forming virus DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Eisenbeis C F
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Singh H
Storb U
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.10.6452-6461.1993
Published
1993-10-00
Pages
6452-61
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364704
Subset
IM
Grants
NIGMS NIH HHS · GM07281 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.10.6452-6461.1993 PMC Full Text View journal details
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