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PMID: 8502565 Published · ppublish English Journal Article

The Rb97D gene encodes a potential RNA-binding protein required for spermatogenesis in Drosophila.

Nucleic acids research ·Vol. 21 ·No. 9 ·1993-05-11 ·Pages 2229-35

Karsch-Mizrachi I, Haynes SR

Abstract

Many proteins that bind RNA contain a common RNA-binding domain, the RNP motif. We have been studying two Drosophila RNP motif proteins, Hrb98DE and Hrb87F, which are hnRNA-binding proteins. We report here the characterization of the Rb97D gene, which encodes a protein that is closely related to the Hrb proteins in the RNP motif domain, but has a distinctive proline-rich C-terminal domain. The gene is located at 97D on the right arm of the third chromosome, near the rough gene. Multiple transcripts from the Rb97D gene are present at varying levels throughout development. The transcripts are generated by alternative processing in the coding and 3' untranslated regions, and can encode two protein isoforms. Analysis of a mutant containing a P element inserted into the 5' untranslated region of the gene demonstrates that Rb97D is required for male fertility. Possible models for the function of Rb97D in testes are discussed.

Related Genes
Rb97D
MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Drosophila Drosophila Proteins Male Molecular Sequence Data RNA-Binding Proteins/genetics Sequence Homology, Amino Acid Spermatogenesis/genetics
Chemicals
Drosophila Proteins RNA-Binding Proteins Rb97D protein, Drosophila DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Karsch-Mizrachi I
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Haynes S R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.9.2229
Published
1993-05-11
Pages
2229-35
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309489
Subset
IM
Databases
GENBANK
L02106
External Links
PubMed source DOI: 10.1093/nar/21.9.2229 PMC Full Text View journal details
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