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PMID: 8567721 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

VAMP/synaptobrevin isoforms 1 and 2 are widely and differentially expressed in nonneuronal tissues.

The Journal of cell biology ·Vol. 132 ·No. 1-2 ·1996-01-00 ·Pages 167-79

Rossetto O, Gorza L, Schiavo G, Schiavo N, Scheller RH, Montecucco C

Abstract

VAMP/synaptobrevin is part of the synaptic vesicle docking and fusion complex and plays a central role in neuroexocytosis. Two VAMP (vesicle-associated membrane protein) isoforms are expressed in the nervous system and are differently distributed among the specialized parts of the tissue. Here, VAMP-1 and -2 are shown to be present in all rat tissues tested, including kidney, adrenal gland, liver, pancreas, thyroid, heart, and smooth muscle. The two isoforms are differentially expressed in various tissues and their level may depend on differentiation. VAMP-1 is restricted to exocrine pancreas and to kidney tubular cells, whereas VAMP-2 is the predominant isoform present in Langerhans islets and in glomerular cells. Both isoforms show a patchy vesicular intracellular distribution in confocal microscopy. The present results provide evidence for the importance of neuronal VAMP proteins in the physiology of all cells.

MeSH Terms
Animals Blotting, Northern Blotting, Western Cell Compartmentation Fluorescent Antibody Technique Gene Expression In Situ Hybridization Male Membrane Proteins/biosynthesis,genetics,isolation & purification R-SNARE Proteins RNA, Messenger/isolation & purification Rats Rats, Wistar Tissue Distribution
Chemicals
Membrane Proteins R-SNARE Proteins RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rossetto O
Dipartimento di Scienze Biomediche, Università di Padova, Italy.
Gorza L
Schiavo G
Schiavo N
Scheller R H
Montecucco C
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.132.1.167
Published
1996-01-00
Pages
167-79
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120699
Subset
IM
Grants
Telethon · 473 · Italy
External Links
PubMed source DOI: 10.1083/jcb.132.1.167 PMC Full Text View journal details
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