Home LiteratureArticle Details
PMID: 8627682 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep.

Journal of virology ·Vol. 70 ·No. 3 ·1996-03-00 ·Pages 1618-23

Palmarini M, Cousens C, Dalziel RG, Bai J, Stedman K, DeMartini JC, Sharp JM

Abstract

Sheep pulmonary adenomatosis ([SPA] ovine pulmonary carcinoma) is a transmissible lung cancer of sheep that has been associated etiologically with a type D- and B-related retrovirus (jaagsiekte retrovirus (JSRV]). To date it has been impossible to cultivate JSRV in vitro and therefore to demonstrate the etiology of SPA by a classical approach. In addition, the presence of 15 to 20 copies of endogenous JSRV-related sequences (enJSRV) has hampered studies at the molecular level. The aim of this study was to investigate whether the expression of exogenous JSRV was specifically associated with neoplasia in SPA-affected animals. Initially, we found that enJSRVs were transcribed in a wide variety of normal sheep tissues. Then, by sequencing part of the gag gene of enJSRV we established a ScaI restriction site in gag as a molecular marker for the exogenous form of JSRV. Restriction enzyme digestion of PCR products obtained from the amplification of cDNA from a total of 65 tissues collected from SPA-affected and unaffected control sheep revealed that the exogenous form of JSRV was exclusively and consistently present in tumor tissues and lung secretions of the affected animals. In addition, exogenous JSRV provirus was detected only in DNA from SPA tumors and not from nontumor tissues of the same animals. This study has demonstrated clearly that the exogenous form of JSRV is specifically associated with SPA tumors.

MeSH Terms
Animals Base Sequence Betaretrovirus/isolation & purification,physiology DNA, Viral Dogs Equidae Genes, gag Lung Neoplasms/pathology,veterinary,virology Molecular Sequence Data Polymerase Chain Reaction Pulmonary Adenomatosis, Ovine/transmission,virology RNA, Messenger/metabolism Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Sheep
Chemicals
DNA, Viral RNA, Messenger
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Palmarini M
Moredun Research Institute, Edinburgh, United Kingdom.
Cousens C
Dalziel R G
Bai J
Stedman K
DeMartini J C
Sharp J M
References (38)
38 references, click to expand
  1. Pulmonary adenomatosis of sheep (jaagsiekte). I. Ultrastructure of the tumor.
    J Natl Cancer Inst. 1971 Mar;46(3):525-37 PMID: 4100700
  2. Virological events leading to spontaneous AKR thymomas.
    J Virol. 1991 Mar;65(3):1273-85 PMID: 1847454
  3. Aetiology of jaagsiekte: transmission by means of subcellular fractions and evidence for the involvement of a retrovirus.
    Onderstepoort J Vet Res. 1980 Dec;47(4):275-80 PMID: 6164973
  4. Sheep lung carcinoma: an endemic analogue of a sporadic human neoplasm.
    J Natl Cancer Inst. 1982 Oct;69(4):747-9 PMID: 6288989
  5. The high leukemogenic potential of Gross passage A murine leukemia virus maps in the region of the genome corresponding to the long terminal repeat and to the 3' end of env.
    J Virol. 1983 Jul;47(1):24-32 PMID: 6306276
  6. Further evidence for a retrovirus as the aetiological agent of sheep pulmonary adenomatosis (jaagsiekte).
    Vet Microbiol. 1983 Jun;8(3):237-49 PMID: 6193627
  7. Sheep pulmonary adenomatosis: demonstration of a protein which cross-reacts with the major core proteins of Mason-Pfizer monkey virus and mouse mammary tumour virus.
    J Gen Virol. 1983 Oct;64 (Pt 10):2323-7 PMID: 6311958
  8. Nuclear factor 1 activates the feline leukemia virus long terminal repeat but is posttranscriptionally down-regulated in leukemia cell lines.
    J Virol. 1991 Apr;65(4):1991-9 PMID: 1848314
  9. SL3-3 enhancer factor 1 transcriptional activators are required for tumor formation by SL3-3 murine leukemia virus.
    J Virol. 1991 Aug;65(8):4177-81 PMID: 1649324
  10. Nucleotide sequence of the jaagsiekte retrovirus, an exogenous and endogenous type D and B retrovirus of sheep and goats.
    J Virol. 1992 Aug;66(8):4930-9 PMID: 1629959
  11. Evidence for retroviral capsid and nucleocapsid antigens in ovine pulmonary carcinoma.
    Virus Res. 1992 Sep 15;25(3):159-67 PMID: 1332272
  12. Animal models of retrovirus-associated malignancies.
    Vet Pathol. 1992 Nov;29(6):572-8 PMID: 1360178
  13. Rising incidence of bronchioloalveolar lung carcinoma and its unique clinicopathologic features.
    Cancer. 1994 Feb 15;73(4):1163-70 PMID: 8313318
  14. Defective endogenous proviruses are expressed in feline lymphoid cells: evidence for a role in natural resistance to subgroup B feline leukemia viruses.
    J Virol. 1994 Apr;68(4):2151-60 PMID: 8138999
  15. Analysis of a type D retroviral capsid gene expressed in ovine pulmonary carcinoma and present in both affected and unaffected sheep genomes.
    Virology. 1994 Jul;202(1):480-4 PMID: 8009860
  16. Coexpression of exogenous and endogenous mouse mammary tumor virus RNA in vivo results in viral recombination and broadens the virus host range.
    J Virol. 1994 Aug;68(8):5019-26 PMID: 8035502
  17. Long terminal repeat enhancer core sequences in proviruses adjacent to c-myc in T-cell lymphomas induced by a murine retrovirus.
    J Virol. 1995 Jan;69(1):446-55 PMID: 7983741
  18. HPRS-103 (exogenous avian leukosis virus, subgroup J) has an env gene related to those of endogenous elements EAV-0 and E51 and an E element found previously only in sarcoma viruses.
    J Virol. 1995 Feb;69(2):779-84 PMID: 7815543
  19. Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus.
    J Gen Virol. 1995 Nov;76 ( Pt 11):2731-7 PMID: 7595380
  20. Localization of the leukemogenic determinants of SL3-3, an ecotropic, XC-positive murine leukemia virus of AKR mouse origin.
    J Virol. 1983 Aug;47(2):317-28 PMID: 6312068
  21. Rapid transmission of sheep pulmonary adenomatosis (jaagsiekte) in young lambs. Brief report.
    Arch Virol. 1983;78(1-2):89-95 PMID: 6197047
  22. Lung cancer in nonsmokers.
    Cancer. 1984 Mar 1;53(5):1214-21 PMID: 6692309
  23. Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat.
    Nature. 1984 Mar 29-Apr 4;308(5958):467-70 PMID: 6323995
  24. The tandem direct repeats within the long terminal repeat of murine leukemia viruses are the primary determinant of their leukemogenic potential.
    J Virol. 1984 Dec;52(3):945-52 PMID: 6092722
  25. Bronchoalveolar cell carcinoma of the lung.
    Ann Thorac Surg. 1986 Jun;41(6):652-6 PMID: 3013107
  26. The localization of a Mason-Pfizer monkey virus-related antigen in jaagsiekte tumour tissue and cell lines.
    Onderstepoort J Vet Res. 1986 Jun;53(2):87-91 PMID: 3523361
  27. Experimental transmission of sheep pulmonary adenomatosis to a goat.
    Vet Rec. 1986 Sep 6;119(10):245 PMID: 3765304
  28. Experimental coinduction of type D retrovirus-associated pulmonary carcinoma and lentivirus-associated lymphoid interstitial pneumonia in lambs.
    J Natl Cancer Inst. 1987 Jul;79(1):167-77 PMID: 3474445
  29. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  30. Sheep pulmonary adenomatosis: a contagious tumour and its cause.
    Cancer Surv. 1987;6(1):73-83 PMID: 3319134
  31. Lesions and retroviruses associated with naturally occurring ovine pulmonary carcinoma (sheep pulmonary adenomatosis).
    Vet Pathol. 1988 Jan;25(1):58-66 PMID: 2830697
  32. The pathology of lung cancer--changing concepts and newer diagnostic techniques.
    Semin Oncol. 1988 Jun;15(3):215-25 PMID: 2837828
  33. The etiology and pathogenesis of ovine pulmonary carcinoma (sheep pulmonary adenomatosis).
    Vet Microbiol. 1988 Jul;17(3):219-36 PMID: 3055655
  34. Retrovirus-associated ovine pulmonary carcinoma (sheep pulmonary adenomatosis) and lymphoid interstitial pneumonia. I. Lesion development and age susceptibility.
    Vet Pathol. 1988 Nov;25(6):475-83 PMID: 3212891
  35. Cancer statistics, 1990.
    CA Cancer J Clin. 1990 Jan-Feb;40(1):9-26 PMID: 2104569
  36. Mutation of the core or adjacent LVb elements of the Moloney murine leukemia virus enhancer alters disease specificity.
    Genes Dev. 1990 Feb;4(2):233-42 PMID: 2338244
  37. Significant changes in the distribution of histologic types of lung cancer. A review of 4928 cases.
    Cancer. 1990 May 15;65(10):2361-7 PMID: 2161283
  38. Experimental production of sheep pulmonary adenomatosis (Jaagsiekte).
    Nature. 1976 Nov 11;264(5582):183-5 PMID: 186718
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.3.1618-1623.1996
Published
1996-03-00
Pages
1618-23
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189985
Subset
IM
Databases
GENBANK
X92180, X92181, X92182, Z49800, Z66531, Z66532, Z66533
External Links
PubMed source DOI: 10.1128/JVI.70.3.1618-1623.1996 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2