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PMID: 8657574 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Gene structure and characterization of the murine homologue of the B cell-specific transcriptional coactivator OBF-1.

Nucleic acids research ·Vol. 24 ·No. 10 ·1996-05-15 ·Pages 1913-20

Schubart DB, Sauter P, Massa S, Friedl EM, Schwarzenbach H, Matthias P

Abstract

The B cell-specific activity of immunoglobulin (Ig) gene promoters is to a large extent mediated by the conserved octamer motif ATTTGCAT. This requires the DNA binding octamer factors Oct-1 and/or Oct-2, as well as an additional B cell-restricted non-DNA binding cofactor. We recently cloned such a coactivator specific for Oct-1 or Oct-2 from human B cells and called it OBF-1. Here we report the isolation and characterization of the murine homologue. Full-length cDNA clones as well as genomic clones were isolated and the gene structure was determined. The deduced protein sequence shows that the mouse protein has an identical length, is likewise proline rich and shows 89% overall identity to the human protein. The OBF-1 gene is expressed in a very highly B cell-specific manner and is transcribed in cells representative of all stages of B cell differentiation, including the earliest ones. We show that OBF-1 interacts in the absence of DNA with the POU domain of Oct-1 or Oct-2 and also with the general transcription factors TBP and TFIIB. Furthermore, we demonstrate that although OBF-1 efficiently activates promoter octamer sites, it does not activate enhancer octamer sites.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/cytology,metabolism Base Sequence Cell Differentiation DNA/chemistry DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Gene Expression Homeodomain Proteins/metabolism Host Cell Factor C1 Humans Infant, Newborn Mice Molecular Sequence Data Octamer Transcription Factor-1 Octamer Transcription Factor-2 POU Domain Factors Promoter Regions, Genetic Sequence Homology Trans-Activators/chemistry,genetics Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins HCFC1 protein, human Hcfc1 protein, mouse Homeodomain Proteins Host Cell Factor C1 Octamer Transcription Factor-1 Octamer Transcription Factor-2 POU Domain Factors POU2AF1 protein, human POU2F1 protein, human POU2F2 protein, human Pou2af1 protein, mouse Pou2f1 protein, mouse Pou2f2 protein, mouse Trans-Activators Transcription Factors DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schubart D B
Friedrich Miescher Institute, Basel, Switzerland.
Sauter P
Massa S
Friedl E M
Schwarzenbach H
Matthias P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.10.1913
Published
1996-05-15
Pages
1913-20
Language
English
Region
England
NLM ID
0411011
PMCID
PMC145881
Subset
IM
Databases
GENBANK
U43788
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1093/nar/24.10.1913 PMC Full Text View journal details
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