Home LiteratureArticle Details
PMID: 8892890 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel C/EBP beta-YY1 complex controls the cell-type-specific activity of the human papillomavirus type 18 upstream regulatory region.

Journal of virology ·Vol. 70 ·No. 11 ·1996-11-00 ·Pages 7695-705

Bauknecht T, See RH, Shi Y

Abstract

The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls viral gene transcription in a cell-type-specific manner. The HPV-18 URR is active in HeLa cells but inactive in HepG2 cells. The activating activity of YY1 in HeLa cells is dependent on its functional interactions with the switch region which is critical for the HPV-18 URR activity in HeLa cells. Here, we show that a protein complex composed of C/EBP beta and YY1 binds the switch region which is detected only in HeLa cells, not in HepG2 cells. Transfection of C/EBP beta into HepG2 cells restored the formation of the C/EBP beta-YY1-switch region complex, accompanied by increased transcription directed by the HPV-18 URR. Mutations in the switch region that abolished the complex formation also abrogated C/EBP beta-induced transcriptional activation. This provides a strong correlation between the binding of the C/EBP beta-YY1 complex to the switch region and cell-type-specific URR activity. Taken together, we have identified a novel C/EBP beta-YY1 complex that binds the switch region and contributes to cell-type-specific HPV-18 URR activity.

MeSH Terms
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins/metabolism Erythroid-Specific DNA-Binding Factors HeLa Cells Humans Nuclear Proteins/metabolism Papillomaviridae/genetics Regulatory Sequences, Nucleic Acid Transcription Factors/metabolism Tumor Cells, Cultured YY1 Transcription Factor
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Nuclear Proteins Transcription Factors YY1 Transcription Factor YY1 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bauknecht T
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
See R H
Shi Y
References (81)
81 references, click to expand
  1. Interleukin 6 induces secretion of IgG1 by coordinated transcriptional activation and differential mRNA accumulation.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):8024-8 PMID: 2813375
  2. Scissors-grip model for DNA recognition by a family of leucine zipper proteins.
    Science. 1989 Nov 17;246(4932):911-6 PMID: 2683088
  3. HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes.
    EMBO J. 1989 Dec 1;8(12):3905-10 PMID: 2555178
  4. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product.
    EMBO J. 1989 Dec 20;8(13):4099-105 PMID: 2556261
  5. Association of human papillomavirus types 16 and 18 E6 proteins with p53.
    Science. 1990 Apr 6;248(4951):76-9 PMID: 2157286
  6. Repression of the human papillomavirus type 18 enhancer by the cellular transcription factor Oct-1.
    J Virol. 1991 Oct;65(10):5613-8 PMID: 1654457
  7. Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein.
    Cell. 1991 Oct 18;67(2):377-88 PMID: 1655281
  8. cAMP stimulates the C/EBP-related transcription factor rNFIL-6 to trans-locate to the nucleus and induce c-fos transcription.
    Genes Dev. 1991 Oct;5(10):1754-66 PMID: 1655570
  9. A keratinocyte-specific transcription factor, KRF-1, interacts with AP-1 to activate expression of human papillomavirus type 18 in squamous epithelial cells.
    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9102-6 PMID: 1656460
  10. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA.
    Cell. 1991 Nov 1;67(3):569-79 PMID: 1934061
  11. Delta, a transcription factor that binds to downstream elements in several polymerase II promoters, is a functionally versatile zinc finger protein.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9799-803 PMID: 1946404
  12. Isolation of a candidate repressor/activator, NF-E1 (YY-1, delta), that binds to the immunoglobulin kappa 3' enhancer and the immunoglobulin heavy-chain mu E1 site.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9804-8 PMID: 1946405
  13. Viruses in human cancers.
    Science. 1991 Nov 22;254(5035):1167-73 PMID: 1659743
  14. Cloning of a negative transcription factor that binds to the upstream conserved region of Moloney murine leukemia virus.
    Mol Cell Biol. 1992 Jan;12(1):38-44 PMID: 1309593
  15. NF-IL6, a member of the C/EBP family, regulates E1A-responsive promoters in the absence of E1A.
    J Virol. 1992 Feb;66(2):1021-30 PMID: 1309887
  16. A member of the C/EBP family, NF-IL6 beta, forms a heterodimer and transcriptionally synergizes with NF-IL6.
    Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1473-6 PMID: 1741402
  17. CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription.
    Genes Dev. 1992 Mar;6(3):439-53 PMID: 1547942
  18. Fos and Jun repress transcription activation by NF-IL6 through association at the basic zipper region.
    Mol Cell Biol. 1994 Jan;14(1):268-76 PMID: 8264594
  19. A novel cis-acting element controlling the rat CYP2D5 gene and requiring cooperativity between C/EBP beta and an Sp1 factor.
    Mol Cell Biol. 1994 Feb;14(2):1383-94 PMID: 8289814
  20. Three levels of functional interaction determine the activity of CCAAT/enhancer binding protein-alpha on the serum albumin promoter.
    Genes Dev. 1994 Feb 1;8(3):350-62 PMID: 8314088
  21. The E6/E7 promoter of extrachromosomal HPV16 DNA in cervical cancers escapes from cellular repression by mutation of target sequences for YY1.
    EMBO J. 1994 Mar 15;13(6):1460-6 PMID: 8137827
  22. Identification of a C/EBP-Rel complex in avian lymphoid cells.
    Mol Cell Biol. 1994 Oct;14(10):6635-46 PMID: 7935382
  23. Transcription factors NF-IL6 and CREB recognize a common essential site in the human prointerleukin 1 beta gene.
    Mol Cell Biol. 1994 Nov;14(11):7285-97 PMID: 7935442
  24. Novel mechanism of C/EBP beta (NF-M) transcriptional control: activation through derepression.
    Genes Dev. 1994 Nov 15;8(22):2781-91 PMID: 7958933
  25. A switch region determines the cell type-specific positive or negative action of YY1 on the activity of the human papillomavirus type 18 promoter.
    J Virol. 1995 Jan;69(1):1-12 PMID: 7983700
  26. An analysis of genes regulated by the multi-functional transcriptional regulator Yin Yang-1.
    Nucleic Acids Res. 1994 Dec 11;22(24):5151-5 PMID: 7816599
  27. Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins.
    Genes Dev. 1995 Jan 15;9(2):168-81 PMID: 7531665
  28. Adenovirus E1A proteins interact with the cellular YY1 transcription factor.
    J Virol. 1995 Mar;69(3):1628-36 PMID: 7853498
  29. Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300.
    Genes Dev. 1995 May 15;9(10):1188-98 PMID: 7758944
  30. CRP2 (C/EBP beta) contains a bipartite regulatory domain that controls transcriptional activation, DNA binding and cell specificity.
    EMBO J. 1995 Jul 3;14(13):3170-83 PMID: 7621830
  31. C/EBP proteins activate transcription from the human immunodeficiency virus type 1 long terminal repeat in macrophages/monocytes.
    J Virol. 1995 Sep;69(9):5337-44 PMID: 7636977
  32. Repression of the interleukin-6 promoter by estrogen receptor is mediated by NF-kappa B and C/EBP beta.
    Mol Cell Biol. 1995 Sep;15(9):4971-9 PMID: 7651415
  33. Retinoblastoma protein directly interacts with and activates the transcription factor NF-IL6.
    Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):465-9 PMID: 8552662
  34. Transcriptional control of human papillomavirus type 18 oncogene expression in different cell lines: role of transcription factor YY1.
    Virus Genes. 1995;11(1):53-8 PMID: 8808335
  35. Human cervical and foreskin epithelial cells immortalized by human papillomavirus DNAs exhibit dysplastic differentiation in vivo.
    Cancer Res. 1990 Jun 15;50(12):3709-15 PMID: 1692766
  36. A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family.
    EMBO J. 1990 Jun;9(6):1897-906 PMID: 2112087
  37. A member of the activator protein 1 family found in keratinocytes but not in fibroblasts required for transcription from a human papillomavirus type 18 promoter.
    J Virol. 1990 Oct;64(10):4792-8 PMID: 2168967
  38. The E6/E7 promoter of human papillomavirus type 16 is activated in the absence of E2 proteins by a sequence-aberrant Sp1 distal element.
    J Virol. 1990 Nov;64(11):5577-84 PMID: 2170687
  39. IL-6DBP, a nuclear protein involved in interleukin-6 signal transduction, defines a new family of leucine zipper proteins related to C/EBP.
    Cell. 1990 Nov 2;63(3):643-53 PMID: 2171780
  40. Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family.
    Mol Cell Biol. 1990 Dec;10(12):6642-53 PMID: 1701020
  41. LAP, a novel member of the C/EBP gene family, encodes a liver-enriched transcriptional activator protein.
    Genes Dev. 1990 Sep;4(9):1541-51 PMID: 2253878
  42. Calcium-regulated phosphorylation within the leucine zipper of C/EBP beta.
    Science. 1992 Apr 17;256(5055):370-3 PMID: 1314426
  43. Two AP1 sites binding JunB are essential for human papillomavirus type 18 transcription in keratinocytes.
    J Virol. 1992 Jun;66(6):3740-8 PMID: 1316480
  44. Efficiency of binding the retinoblastoma protein correlates with the transforming capacity of the E7 oncoproteins of the human papillomaviruses.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4442-6 PMID: 1316608
  45. The p50 subunit of NF-kappa B associates with the NF-IL6 transcription factor.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8145-9 PMID: 1518839
  46. Displacement of BrdUrd-induced YY1 by serum response factor activates skeletal alpha-actin transcription in embryonic myoblasts.
    Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9814-8 PMID: 1409704
  47. Identification of a negative regulatory domain in the human papillomavirus type 18 promoter: interaction with the transcriptional repressor YY1.
    EMBO J. 1992 Dec;11(12):4607-17 PMID: 1330541
  48. NF-IL6 represses early gene expression of human papillomavirus type 16 through binding to the noncoding region.
    J Virol. 1993 Feb;67(2):1058-66 PMID: 8380454
  49. Activation of human papillomavirus type 18 E6-E7 oncogene expression by transcription factor Sp1.
    Nucleic Acids Res. 1992 Dec 25;20(24):6701-6 PMID: 1336181
  50. A nuclear factor for interleukin-6 expression (NF-IL6) and the glucocorticoid receptor synergistically activate transcription of the rat alpha 1-acid glycoprotein gene via direct protein-protein interaction.
    Mol Cell Biol. 1993 Mar;13(3):1854-62 PMID: 8441418
  51. Convergent regulation of NF-IL6 and Oct-1 synthesis by interleukin-6 and retinoic acid signaling in embryonal carcinoma cells.
    Mol Cell Biol. 1993 Apr;13(4):2515-23 PMID: 8455626
  52. Phosphorylation at threonine-235 by a ras-dependent mitogen-activated protein kinase cascade is essential for transcription factor NF-IL6.
    Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2207-11 PMID: 8384717
  53. The NF-M transcription factor is related to C/EBP beta and plays a role in signal transduction, differentiation and leukemogenesis of avian myelomonocytic cells.
    EMBO J. 1993 Apr;12(4):1321-32 PMID: 8467792
  54. C/ATF, a member of the activating transcription factor family of DNA-binding proteins, dimerizes with CAAT/enhancer-binding proteins and directs their binding to cAMP response elements.
    Proc Natl Acad Sci U S A. 1993 May 15;90(10):4679-83 PMID: 8506317
  55. Functional and physical associations between NF-kappa B and C/EBP family members: a Rel domain-bZIP interaction.
    Mol Cell Biol. 1993 Jul;13(7):3964-74 PMID: 8321203
  56. Transactivation by NF-IL6/LAP is enhanced by phosphorylation of its activation domain.
    Nature. 1993 Aug 5;364(6437):544-7 PMID: 8336793
  57. CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.
    Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8219-23 PMID: 8367486
  58. Distinct mechanisms for regulation of the interleukin-8 gene involve synergism and cooperativity between C/EBP and NF-kappa B.
    Mol Cell Biol. 1993 Nov;13(11):7191-8 PMID: 8413306
  59. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53.
    Cell. 1990 Dec 21;63(6):1129-36 PMID: 2175676
  60. CCAAT-enhancer binding protein: a component of a differentiation switch.
    Science. 1991 Jan 18;251(4991):288-92 PMID: 1987644
  61. Interleukin 6 enhances a cellular activity that functionally substitutes for E1A protein in transactivation.
    Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6472-6 PMID: 1830663
  62. Human papillomaviruses in the pathogenesis of anogenital cancer.
    Virology. 1991 Sep;184(1):9-13 PMID: 1651607
  63. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells.
    Genes Dev. 1991 Sep;5(9):1538-52 PMID: 1840554
  64. A family of C/EBP-related proteins capable of forming covalently linked leucine zipper dimers in vitro.
    Genes Dev. 1991 Sep;5(9):1553-67 PMID: 1884998
  65. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  66. A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3812-5 PMID: 6304740
  67. Structure and transcription of human papillomavirus sequences in cervical carcinoma cells.
    Nature. 1985 Mar 7-13;314(6006):111-4 PMID: 2983228
  68. Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4680-4 PMID: 3014503
  69. Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes.
    EMBO J. 1986 Sep;5(9):2285-92 PMID: 3023067
  70. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.
    J Virol. 1987 Apr;61(4):962-71 PMID: 3029430
  71. Firefly luciferase gene: structure and expression in mammalian cells.
    Mol Cell Biol. 1987 Feb;7(2):725-37 PMID: 3821727
  72. High-efficiency transformation of mammalian cells by plasmid DNA.
    Mol Cell Biol. 1987 Aug;7(8):2745-52 PMID: 3670292
  73. Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses.
    Genes Dev. 1987 Apr;1(2):133-46 PMID: 2824279
  74. Characterization of primary human keratinocytes transformed by human papillomavirus type 18.
    J Virol. 1988 Jun;62(6):1917-24 PMID: 2452896
  75. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
    Science. 1988 Jun 24;240(4860):1759-64 PMID: 3289117
  76. Human papillomavirus type 16 alters human epithelial cell differentiation in vitro.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7169-73 PMID: 2459699
  77. Isolation of a recombinant copy of the gene encoding C/EBP.
    Genes Dev. 1988 Jul;2(7):786-800 PMID: 2850264
  78. The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product.
    Science. 1989 Feb 17;243(4893):934-7 PMID: 2537532
  79. The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite.
    Science. 1989 Mar 31;243(4899):1681-8 PMID: 2494700
  80. Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers.
    Cancer Res. 1989 Sep 1;49(17):4677-81 PMID: 2547512
  81. The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes.
    J Virol. 1989 Oct;63(10):4417-21 PMID: 2476573
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.11.7695-7705.1996
Published
1996-11-00
Pages
7695-705
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190839
Subset
IM
Grants
NIGMS NIH HHS · GM 53874 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.11.7695-7705.1996 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2