Home LiteratureArticle Details
PMID: 893344 Published · ppublish English Journal Article

Ribosomal suppressors and antisuppressors in Podospora anserina: resistance to cycloheximide.

Journal of bacteriology ·Vol. 131 ·No. 3 ·1977-09-00 ·Pages 876-83

Coppin-Raynal E

Abstract

Informational suppressors and antisuppressors have been previously isolated in Podospora anserina, and a range of exclusively genetic arguments have led to the assumption that they correspond to ribosomal mutations. An in vivo and in vitro comparison of the effect of the ribosomal inhibitor cycloheximide on wildtype and mutant strains described in this paper confirms the ribosomal hypothesis for at least some mutants. Indeed, the four mutants in the AS3 gene were cycloheximide resistant, and their ribosomes were found to be resistant when analyzed by polyuridyl-directed polyphenylalanine systhesis. On the other hand, ribosomes from two su 1 mutants were hypersensitive to the drug.

MeSH Terms
Ascomycota/drug effects Cell-Free System Cycloheximide/pharmacology Drug Resistance, Microbial Mutation Peptide Biosynthesis Phenylalanine/metabolism Poly U/metabolism Ribosomes/drug effects,metabolism Suppression, Genetic Xylariales/drug effects,growth & development,metabolism
Chemicals
Poly U Phenylalanine Cycloheximide
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Coppin-Raynal E
References (22)
22 references, click to expand
  1. Genetic studies on cycloheximide-resistant strains of Schizosaccharomyces pombe.
    Heredity (Edinb). 1976 Oct;37(2):179-91 PMID: 1068110
  2. Genetic evidence of ribosomal antisuppressors in Podospora anserina.
    Mol Gen Genet. 1976 Sep 23;147(3):299-306 PMID: 967160
  3. Mutation in Saccharomyces cerevisiae conferring streptomycin and cold sensitivity by affecting ribosome formation and function.
    J Bacteriol. 1974 May;118(2):319-28 PMID: 4133351
  4. Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli.
    Nature. 1969 Apr 26;222(5191):333-9 PMID: 4181187
  5. Genetic analysis of antisuppressor mutants in the fission yeast Schizosaccharomyces pombe.
    Mol Gen Genet. 1976 Dec 31;142(4):251-61 PMID: 1272244
  6. Role of ribosomes in cycloheximide resistance of Neurospora mutants.
    Mol Gen Genet. 1973 Aug 28;124(4):359-63 PMID: 4270517
  7. Neurospora crassa cytoplasmic ribosomes: isolation and characterization of a cold-sensitive mutant defective in ribosome biosynthesis.
    J Bacteriol. 1974 Nov;120(2):666-71 PMID: 4281772
  8. Functional interdependence of 50S and 30S ribosomal subunits.
    Mol Gen Genet. 1974;135(1):11-8 PMID: 4280506
  9. Cryptopleurine resistance: genetic locus for a 40S ribosomal component in Saccharomyces cerevisiae.
    J Bacteriol. 1974 Dec;120(3):1308-14 PMID: 4612014
  10. Cold-sensitive mutants in Aspergillus nidulans. II. Mutations affecting ribosome production.
    Mol Gen Genet. 1974;134(2):115-32 PMID: 4617158
  11. Modification of ribosomes in cryptopleurine-resistant mutants of yeast.
    J Bacteriol. 1973 Nov;116(2):818-22 PMID: 4583252
  12. Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins.
    Mol Gen Genet. 1973 Dec 20;127(2):175-89 PMID: 4589347
  13. Trichodermin resistance--mutation affecting eukaryotic ribosomes.
    Nature. 1974 Apr 5;248(448):535-6 PMID: 4596184
  14. Alteration of a 30S ribosomal protein accompanying the ram mutation in Escherichia coli.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2263-7 PMID: 4943794
  15. A ribosomal ambiguity mutation.
    J Mol Biol. 1969 Jan 14;39(1):95-112 PMID: 4938819
  16. A new gene for ribosomal restriction in Escherichia coli.
    Mol Gen Genet. 1975;137(1):73-78 PMID: 1101029
  17. Direct selection of mutants restricting efficiency of suppression and misreading levels in E. coli B.
    Mol Gen Genet. 1975;137(4):277-87 PMID: 1102920
  18. The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes.
    J Biol Chem. 1971 Jan 10;246(1):174-81 PMID: 5541758
  19. [Selection of actidion-resistant mutants in Neurospora crassa and their genetical and biochemical analysis].
    Mol Gen Genet. 1970;106(2):180-94 PMID: 5415255
  20. Cycloheximide resistance in yeast: a property of the 60s ribosomal subunit.
    Biochem Biophys Res Commun. 1967 Dec 15;29(5):696-704 PMID: 6077806
  21. Modified ribosomes conferring resistance to cycloheximide in mutants of Saccharomyces cerevisiae.
    J Mol Biol. 1967 Jun 14;26(2):347-50 PMID: 6034778
  22. Site of action of cycloheximide in cells of Saccharomyces pastorianus. 3. Further studies on the mechanism of action and the mechanism of resistance in saccharomyces species.
    Biochim Biophys Acta. 1965 Aug 10;103(4):558-67 PMID: 5859844
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.131.3.876-883.1977
Published
1977-09-00
Pages
876-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235544
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.131.3.876-883.1977 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2