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PMID: 8943372 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex.

Molecular and cellular biology ·Vol. 16 ·No. 12 ·1996-12-00 ·Pages 7161-72

Schneiter R, Hitomi M, Ivessa AS, Fasch EV, Kohlwein SD, Tartakoff AM

Abstract

The conditional mRNA transport mutant of Saccharomyces cerevisiae, acc1-7-1 (mtr7-1), displays a unique alteration of the nuclear envelope. Unlike nucleoporin mutants and other RNA transport mutants, the intermembrane space expands, protuberances extend from the inner membrane into the intermembrane space, and vesicles accumulate in the intermembrane space. MTR7 is the same gene as ACC1, encoding acetyl coenzyme A (CoA) carboxylase (Acc1p), the rate-limiting enzyme of de novo fatty acid synthesis. Genetic and biochemical analyses of fatty acid synthesis mutants and acc1-7-1 indicate that the continued synthesis of malonyl-CoA, the enzymatic product of acetyl-CoA carboxylase, is required for an essential pathway which is independent from de novo synthesis of fatty acids. We provide evidence that synthesis of very-long-chain fatty acids (C26 atoms) is inhibited in acc1-7-1, suggesting that very-long-chain fatty acid synthesis is required to maintain a functional nuclear envelope.

MeSH Terms
Acetyl-CoA Carboxylase/genetics,metabolism Fatty Acids/genetics,metabolism Gene Expression Regulation, Fungal Membrane Proteins/genetics,metabolism Mutation Nuclear Proteins/genetics,metabolism RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Fatty Acids Membrane Proteins Nuclear Proteins RNA, Fungal RNA, Messenger Acetyl-CoA Carboxylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schneiter R
Institut für Biochemie und Lebensmittelchemie, Technische UniversitätGraz, Austria.
Hitomi M
Ivessa A S
Fasch E V
Kohlwein S D
Tartakoff A M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.12.7161
Published
1996-12-00
Pages
7161-72
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231720
Subset
IM
Grants
NIDDK NIH HHS · DK27651 · United States
NIDDK NIH HHS · DK38181 · United States
NIGMS NIH HHS · GM46569 · United States
External Links
PubMed source DOI: 10.1128/MCB.16.12.7161 PMC Full Text View journal details
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