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PMID: 8972193 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA.

Molecular and cellular biology ·Vol. 17 ·No. 1 ·1997-01-00 ·Pages 135-44

Ernst RK, Bray M, Rekosh D, Hammarskjöld ML

Abstract

A common feature of gene expression in all retroviruses is that unspliced, intron-containing RNA is exported to the cytoplasm despite the fact that cellular RNAs which contain introns are usually restricted to the nucleus. In complex retroviruses, the export of intron-containing RNA is mediated by specific viral regulatory proteins (e.g., human immunodeficiency virus type 1 [HIV-1] Rev) that bind to elements in the viral RNA. However, simpler retroviruses do not encode such regulatory proteins. Here we show that the genome of the simpler retrovirus Mason-Pfizer monkey virus (MPMV) contains an element that serves as an autonomous nuclear export signal for intron-containing RNA. This element is essential for MPMV replication; however, its function can be complemented by HIV-1 Rev and the Rev-responsive element. The element can also facilitate the export of cellular intron-containing RNA. These results suggest that the MPMV element mimics cellular RNA transport signals and mediates RNA export through interaction with endogenous cellular factors.

MeSH Terms
Animals Base Sequence Biological Transport COS Cells Cell Nucleus/virology Chromosome Mapping Cytoplasm/virology Gene Products, env/biosynthesis Gene Products, gag/biosynthesis Gene Products, rev/genetics,physiology HIV-1/genetics Humans Introns Mason-Pfizer monkey virus/genetics,physiology Molecular Sequence Data Nucleic Acid Conformation RNA Splicing RNA, Messenger/metabolism RNA, Viral/chemistry,genetics,metabolism Virus Replication/genetics rev Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, env Gene Products, gag Gene Products, rev RNA, Messenger RNA, Viral rev Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ernst R K
Myles H. Thaler Center for AIDS and Human Retrovirus Research, University of Virginia, Charlottesville 22908, USA.
Bray M
Rekosh D
Hammarskjöld M L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.1.135
Published
1997-01-00
Pages
135-44
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231737
Subset
IM
Databases
GENBANK
K02007, M12349, M19921
External Links
PubMed source DOI: 10.1128/MCB.17.1.135 PMC Full Text View journal details
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