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PMID: 7935458 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human immunodeficiency virus type 1 Rev protein shuttles between the cytoplasm and nuclear compartments.

Molecular and cellular biology ·Vol. 14 ·No. 11 ·1994-11-00 ·Pages 7436-44

Kalland KH, Szilvay AM, Brokstad KA, Saetrevik W, Haukenes G

Abstract

A retroviral regulatory protein, Rev (regulator of virion protein expression), is made in cells infected by human immunodeficiency virus (HIV). Rev is essential for the completion of the retroviral life cycle and interacts with the host cell at some posttranscriptional step in order to express the incompletely spliced HIV mRNAs from which HIV structural proteins are translated. Neither the host cell components nor the mechanisms responsible for this important regulation have been defined. We now report that Rev is a nucleocytoplasmic shuttle protein which is continuously transported between the cytoplasm, the nucleoli, and nucleoplasmic speckles enriched in RNA splicing and processing factors. The results show that Rev has the potential to interfere specifically with the splicing of the HIV pre-mRNA in the nucleoplasm and, next, guide such mRNAs to the cytoplasm for translation.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal Biological Transport, Active/drug effects Cell Compartmentation Cell Nucleus/metabolism Cytoplasm/metabolism Dactinomycin/pharmacology Fluorescent Antibody Technique Gene Products, rev/genetics,immunology,metabolism HIV Antibodies HIV-1/genetics,immunology,metabolism HeLa Cells Humans Molecular Sequence Data Peptide Fragments/genetics,immunology RNA Splicing rev Gene Products, Human Immunodeficiency Virus
Chemicals
Antibodies, Monoclonal Gene Products, rev HIV Antibodies Peptide Fragments rev Gene Products, Human Immunodeficiency Virus Dactinomycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kalland K H
Department of Microbiology and Immunology, Gade Institute, Bergen High Technology Centre, University of Bergen, Norway.
Szilvay A M
Brokstad K A
Saetrevik W
Haukenes G
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47 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.11.7436-7444.1994
Published
1994-11-00
Pages
7436-44
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359279
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.11.7436-7444.1994 PMC Full Text View journal details
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