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PMID: 8972228 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

HSF4, a new member of the human heat shock factor family which lacks properties of a transcriptional activator.

Molecular and cellular biology ·Vol. 17 ·No. 1 ·1997-01-00 ·Pages 469-81

Nakai A, Tanabe M, Kawazoe Y, Inazawa J, Morimoto RI, Nagata K

Abstract

Heat shock transcription factors (HSFs) mediate the inducible transcriptional response of genes that encode heat shock proteins and molecular chaperones. In vertebrates, three related HSF genes (HSF1 to -3) and the respective gene products (HSFs) have been characterized. We report the cloning and characterization of human HSF4 (hHSF4), a novel member of the hHSF family that shares properties with other members of the HSF family yet appears to be functionally distinct. hHSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. hHSF4 is preferentially expressed in the human heart, brain, skeletal muscle, and pancreas. Transient transfection of hHSF4 in HeLa cells, which do not express hHSF4, results in a constitutively active DNA binding trimer which, unlike other members of the HSF family, lacks the properties of a transcriptional activator. Constitutive overexpression of hHSF4 in HeLa cells results in reduced expression of the endogenous hsp70, hsp90, and hsp27 genes. hHSF4 represents a novel hHSF that exhibits tissue-specific expression and functions to repress the expression of genes encoding heat shock proteins and molecular chaperones.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Nucleus/chemistry Chromosome Mapping Chromosomes, Human, Pair 16/genetics Cloning, Molecular DNA, Complementary/genetics DNA-Binding Proteins/analysis,genetics,metabolism Gene Expression Regulation Genes/genetics HeLa Cells Heat Shock Transcription Factors Humans Molecular Sequence Data Organ Specificity RNA, Messenger/analysis Recombinant Fusion Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Trans-Activators Transcription Factors/analysis,genetics,metabolism
Chemicals
DNA, Complementary DNA-Binding Proteins HSF4 protein, human Heat Shock Transcription Factors RNA, Messenger Recombinant Fusion Proteins Trans-Activators Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nakai A
Department of Cell Biology, Kyoto University, Japan. [email protected]
Tanabe M
Kawazoe Y
Inazawa J
Morimoto R I
Nagata K
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40 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.1.469
Email
[email protected]
Published
1997-01-00
Pages
469-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231772
Subset
IM
Databases
GENBANK
D87673
External Links
PubMed source DOI: 10.1128/MCB.17.1.469 PMC Full Text View journal details
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