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PMID: 9064357 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An Epstein-Barr virus-associated superantigen.

The Journal of experimental medicine ·Vol. 184 ·No. 3 ·1996-09-01 ·Pages 971-80

Sutkowski N, Palkama T, Ciurli C, Sekaly RP, Thorley-Lawson DA, Huber BT

Abstract

More than 90% of adults are latently infected with Epstein-Barr virus (EBV), the causative agent of infectious mononucleosis, a self-limiting lymphoproliferative disease characterized by extensive T cell activation. Reactivation of this herpesvirus during immunosuppression is often associated with oncogenesis. These considerations led us to analyze the early events that occur after exposure of the immune system to EBV. Strong major histocompatibility complex (MHC) class II-dependent but not MHC-restricted, T cell proliferation was observed in vitro in response to autologous, lytically infected EBV-transformed B cells. By measuring the appearance of the early activation marker CD69 on individual T cell V beta subsets, we could demonstrate selective activation of human V beta 13- T cells. This was confirmed with murine T cell hybridomas expressing various human BV genes. While EBV- Burkitt's lymphoma cells were nonstimulatory, they induced V beta-restricted T cell activation after EBV infection. EBV specific activation was also demonstrated in cord blood cells, excluding a recall-antigen response. Thus, all of the characteristics of a superantigen-stimulated response are seen, indicating that induction of the EBV lytic cycle is associated with the expression of a superantigen in B cells. A model is presented proposing a role for the superantigen in infection, latency, and oncogenesis.

MeSH Terms
Adult Animals Antigens, CD/analysis Antigens, Differentiation, T-Lymphocyte/analysis Antigens, Viral/analysis B-Lymphocytes/immunology,virology Cell Transformation, Viral HLA-DR Antigens/analysis Herpesvirus 4, Human/immunology Humans Hybridomas/chemistry Infectious Mononucleosis/immunology Lectins, C-Type Mice Models, Biological Molecular Sequence Data Superantigens/analysis T-Lymphocytes/immunology,virology
Chemicals
Antigens, CD Antigens, Differentiation, T-Lymphocyte Antigens, Viral CD69 antigen HLA-DR Antigens Lectins, C-Type Superantigens
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sutkowski N
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Palkama T
Ciurli C
Sekaly R P
Thorley-Lawson D A
Huber B T
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.184.3.971
Published
1996-09-01
Pages
971-80
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192769
Subset
IM
Grants
NIAID NIH HHS · AI 18757 · United States
NCI NIH HHS · CA 31893 · United States
NIAID NIH HHS · R01 AI14910 · United States
Databases
GENBANK
L36092
External Links
PubMed source DOI: 10.1084/jem.184.3.971 PMC Full Text View journal details
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