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PMID: 9190817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A genetic locus involved in iron utilization unique to some Campylobacter strains.

Journal of bacteriology ·Vol. 179 ·No. 12 ·1997-06-00 ·Pages 3997-4002

Guerry P, Perez-Casal J, Yao R, McVeigh A, Trust TJ

Abstract

Two genes involved in iron utilization in Campylobacter coli VC167 T1 have been characterized. The cfrA gene encodes a protein with a predicted Mr of 77,653 which, after processing of the leader sequence, has a predicted Mr of 75,635. This protein has significant sequence identity to siderophore receptors of several bacteria, and site-specific mutants defective in cfrA do not synthesize one of two major iron-repressible outer membrane proteins. An adjacent gene encodes a TonB-like protein; a mutant in this gene lost the ability to utilize hemin, ferrichrome, and enterochelin as iron sources. The cfrA and tonB genes of VC167 T1 hybridized to all strains of C. coli and most strains of C. jejuni examined but did not hybridize to several other strains of C. jejuni, suggesting that the thermophilic campylobacters can be separated into two categories based on the presence of these two iron utilization genes.

MeSH Terms
Amino Acid Sequence Campylobacter/genetics,metabolism Chromosome Mapping DNA, Bacterial/analysis Genes, Bacterial Iron/metabolism Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Hybridization Open Reading Frames
Chemicals
DNA, Bacterial Iron
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Guerry P
Enteric Diseases Program, Naval Medical Research Institute, Bethesda, Maryland 20889, USA. [email protected]
Perez-Casal J
Yao R
McVeigh A
Trust T J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.12.3997-4002.1997
Email
[email protected]
Published
1997-06-00
Pages
3997-4002
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179210
Subset
IM
Databases
GENBANK
U80812
External Links
PubMed source DOI: 10.1128/jb.179.12.3997-4002.1997 PMC Full Text View journal details
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