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PMID: 9312008 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pex14p is a member of the protein linkage map of Pex5p.

The EMBO journal ·Vol. 16 ·No. 18 ·1997-09-15 ·Pages 5491-500

Brocard C, Lametschwandtner G, Koudelka R, Hartig A

Abstract

To identify members of the translocation machinery for peroxisomal proteins, we made use of the two-hybrid system to establish a protein linkage map centered around Pex5p from Saccharomyces cerevisiae, the receptor for the C-terminal peroxisomal targeting signal (PTS1). Among the five interaction partners identified, Pex14p was found to be induced under conditions allowing peroxisome proliferation. Deletion of the corresponding gene resulted in the inability of yeast cells to grow on oleate as well as the absence of peroxisomal structures. The PEX14 gene product of approximately 38 kDa was biochemically and ultrastructurally demonstrated to be a peroxisomal membrane protein, despite the lack of a membrane-spanning domain. This protein was shown to interact with itself, with Pex13p and with both PTS receptors, Pex5p and Pex7p, indicating a central function for the import of peroxisomal matrix proteins, either as a docking protein or as a releasing factor at the organellar membrane.

MeSH Terms
Amino Acid Sequence Base Sequence Carrier Proteins Cloning, Molecular Escherichia coli Fungal Proteins/chemistry,metabolism,ultrastructure Gene Deletion Membrane Proteins/chemistry,metabolism,ultrastructure Membrane Transport Proteins Microbodies/metabolism,ultrastructure Molecular Sequence Data Peroxins Peroxisome-Targeting Signal 1 Receptor Receptors, Cytoplasmic and Nuclear/metabolism Recombinant Proteins/chemistry,metabolism,ultrastructure Repressor Proteins Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Saccharomyces cerevisiae Proteins
Chemicals
Carrier Proteins Fungal Proteins Membrane Proteins Membrane Transport Proteins PEX14 protein, S cerevisiae Peroxins Peroxisome-Targeting Signal 1 Receptor Receptors, Cytoplasmic and Nuclear Recombinant Proteins Repressor Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brocard C
Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Vienna Biocenter, Dr. Bohrgasse 9, A-1030 Wien, Austria.
Lametschwandtner G
Koudelka R
Hartig A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.18.5491
Published
1997-09-15
Pages
5491-500
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170181
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.18.5491 PMC Full Text View journal details
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