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PMID: 9358760 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cellular localization of the chemokine receptor CCR5. Correlation to cellular targets of HIV-1 infection.

The American journal of pathology ·Vol. 151 ·No. 5 ·1997-11-00 ·Pages 1341-51

Rottman JB, Ganley KP, Williams K, Wu L, Mackay CR, Ringler DJ

Abstract

The chemokine receptor CCR5 has recently been described as a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1. In this study, using a panel of monoclonal antibodies specific for human CCR5, we show by immunohistochemistry and flow cytometry that CCR5 is expressed by bone-marrow-derived cells known to be targets for HIV-1 infection, including a subpopulation of lymphocytes and monocyte/macrophages in blood, primary and secondary lymphoid organs, and noninflamed tissues. In the central nervous system, CCR5 is expressed on neurons, astrocytes, and microglia. In other tissues, CCR5 is expressed on epithelium, endothelium, vascular smooth muscle, and fibroblasts. Chronically inflamed tissues contain an increased number of CCR5+ mononuclear cells, and the number of immunoreactive cells is directly associated with a histopathological correlate of inflammatory severity. Collectively, these results suggest that CCR5+ cells are recruited to inflammatory sites and, as such, may facilitate transmission of macrophage-tropic strains of HIV-1.

MeSH Terms
Animals Antibodies, Monoclonal Blood Cells/metabolism Brain/cytology,metabolism HIV Infections/pathology,virology HIV-1 Hippocampus/cytology,metabolism Humans Leukocytes/metabolism Lymph Nodes/cytology,metabolism Macaca Neuroglia/metabolism Neurons/metabolism Receptors, CCR5/metabolism Spleen/cytology,metabolism Thymus Gland/cytology,metabolism Tissue Distribution
Chemicals
Antibodies, Monoclonal Receptors, CCR5
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rottman J B
LeukoSite Inc., Cambridge, MA 02142, USA.
Ganley K P
Williams K
Wu L
Mackay C R
Ringler D J
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1997-11-00
Pages
1341-51
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1858074
Subset
IM
Grants
NINDS NIH HHS · NS35732 · United States
NCRR NIH HHS · RR00168 · United States
External Links
PubMed source PMC Full Text View journal details
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