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PMID: 9488487 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1.

Molecular and cellular biology ·Vol. 18 ·No. 3 ·1998-03-00 ·Pages 1701-10

Chicca JJ, Auble DT, Pugh BF

Abstract

The TATA binding protein (TBP) is a central component of the eukaryotic transcriptional machinery and is the target of positive and negative transcriptional regulators. Here we describe the cloning and biochemical characterization of an abundant human TBP-associated factor (TAF-172) which is homologous to the yeast Mot1 protein and a member of the larger Snf2/Swi2 family of DNA-targeted ATPases. Like Mot1, TAF-172 binds to the conserved core of TBP and uses the energy of ATP hydrolysis to dissociate TBP from DNA (ADI activity). Interestingly, ATP also causes TAF-172 to dissociate from TBP, which has not been previously observed with Mot1. Unlike Mot1, TAF-172 requires both TBP and DNA for maximal (approximately 100-fold) ATPase activation. TAF-172 inhibits TBP-driven RNA polymerase II and III transcription but does not appear to affect transcription driven by TBP-TAF complexes. As it does with Mot1, TFIIA reverses TAF-172-mediated repression of TBP. Together, these findings suggest that human TAF-172 is the functional homolog of yeast Mot1 and uses the energy of ATP hydrolysis to remove TBP (but apparently not TBP-TAF complexes) from DNA.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Adenosine Triphosphate/pharmacology Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA Helicases/genetics,isolation & purification,metabolism DNA, Complementary DNA-Binding Proteins/genetics,metabolism Fungal Proteins/genetics,metabolism HeLa Cells Humans Molecular Sequence Data Recombinant Fusion Proteins/genetics,isolation & purification Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Spodoptera/cytology TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors/genetics,isolation & purification,metabolism Transcription, Genetic
Chemicals
BTAF1 protein, human DNA, Complementary DNA-Binding Proteins Fungal Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors Adenosine Triphosphate Adenosine Triphosphatases MOT1 protein, S cerevisiae DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chicca J J
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park 16802, USA.
Auble D T
Pugh B F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.3.1701
Published
1998-03-00
Pages
1701-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC108885
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055763 · United States
NIGMS NIH HHS · GM47855 · United States
NIGMS NIH HHS · GM55763 · United States
Databases
GENBANK
AF038362
External Links
PubMed source DOI: 10.1128/MCB.18.3.1701 PMC Full Text View journal details
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