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PMID: 9864257 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Response to reactive nitrogen intermediates in Mycobacterium tuberculosis: induction of the 16-kilodalton alpha-crystallin homolog by exposure to nitric oxide donors.

Infection and immunity ·Vol. 67 ·No. 1 ·1999-01-00 ·Pages 460-5

Garbe TR, Hibler NS, Deretic V

Abstract

In contrast to the apparent paucity of Mycobacterium tuberculosis response to reactive oxygen intermediates, this organism has evolved a specific response to nitric oxide challenge. Exposure of M. tuberculosis to NO donors induces the synthesis of a set of polypeptides that have been collectively termed Nox. In this work, the most prominent Nox polypeptide, Nox16, was identified by immunoblotting and by N-terminal sequencing as the alpha-crystallin-related, 16-kDa small heat shock protein, sHsp16. A panel of chemically diverse donors of nitric oxide, with the exception of nitroprusside, induced sHsp16 (Nox16). Nitroprusside, a coordination complex of Fe2+ with a nitrosonium (NO+) ion, induced a 19-kDa polypeptide (Nox19) homologous to the nonheme bacterial ferritins. We conclude that the NO response in M. tuberculosis is dominated by increased synthesis of the alpha-crystallin homolog sHsp16, previously implicated in stationary-phase processes and found in this study to be a major M. tuberculosis protein induced upon exposure to reactive nitrogen intermediates.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/chemistry Crystallins/biosynthesis,chemistry Ferritins/chemistry Gene Expression Regulation, Bacterial/drug effects Heat-Shock Proteins/chemistry Molecular Sequence Data Molecular Weight Mycobacterium tuberculosis/drug effects,genetics,metabolism Nitric Oxide/metabolism Nitric Oxide Donors/metabolism,pharmacology Sequence Homology, Amino Acid
Chemicals
Antigens, Bacterial Crystallins Heat-Shock Proteins Nitric Oxide Donors Nitric Oxide Ferritins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Garbe T R
Department of Microbiology and Immunology, University of Texas Health Science Center San Antonio 78284, USA.
Hibler N S
Deretic V
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.1.460-465.1999
Published
1999-01-00
Pages
460-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC96338
Subset
IM
Grants
NIAID NIH HHS · R01 AI042999 · United States
NIAID NIH HHS · AI42999 · United States
External Links
PubMed source DOI: 10.1128/IAI.67.1.460-465.1999 PMC Full Text View journal details
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