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PMID: 9864345 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Regulation of the sol locus genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 by a putative transcriptional repressor.

Journal of bacteriology ·Vol. 181 ·No. 1 ·1999-01-00 ·Pages 319-30

Nair RV, Green EM, Watson DE, Bennett GN, Papoutsakis ET

Abstract

A gene (orf1, now designated solR) previously identified upstream of the aldehyde/alcohol dehydrogenase gene aad (R. V. Nair, G. N. Bennett, and E. T. Papoutsakis, J. Bacteriol. 176:871-885, 1994) was found to encode a repressor of the sol locus (aad, ctfA, ctfB and adc) genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824. Primer extension analysis identified a transcriptional start site 35 bp upstream of the solR start codon. Amino acid comparisons of SolR identified a potential helix-turn-helix DNA-binding motif in the C-terminal half towards the center of the protein, suggesting a regulatory role. Overexpression of SolR in strain ATCC 824(pCO1) resulted in a solvent-negative phenotype owing to its deleterious effect on the transcription of the sol locus genes. Inactivation of solR in C. acetobutylicum via homologous recombination yielded mutants B and H (ATCC 824 solR::pO1X) which exhibited deregulated solvent production characterized by increased flux towards butanol and acetone formation, earlier induction of aad, lower overall acid production, markedly improved yields of solvents on glucose, a prolonged solvent production phase, and increased biomass accumulation compared to those of the wild-type strain.

MeSH Terms
1-Butanol/metabolism Acetone/metabolism Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Base Sequence Clostridium/genetics,metabolism DNA Primers/genetics Fermentation Gene Expression Genes, Bacterial Molecular Sequence Data Mutation Open Reading Frames Plasmids/genetics Protein Structure, Secondary Repressor Proteins/chemistry,genetics,metabolism Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Bacterial Proteins DNA Primers Repressor Proteins Acetone 1-Butanol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nair R V
Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208, USA.
Green E M
Watson D E
Bennett G N
Papoutsakis E T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.1.319-330.1999
Published
1999-01-00
Pages
319-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC103564
Subset
IM
Databases
GENBANK
L14817
External Links
PubMed source DOI: 10.1128/JB.181.1.319-330.1999 PMC Full Text View journal details
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