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PMID: 10198058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The Yck2 yeast casein kinase 1 isoform shows cell cycle-specific localization to sites of polarized growth and is required for proper septin organization.

Molecular biology of the cell ·Vol. 10 ·No. 4 ·1999-04-00 ·Pages 1077-92

Robinson LC, Bradley C, Bryan JD, Jerome A, Kweon Y, Panek HR

Abstract

Casein kinase 1 protein kinases are ubiquitous and abundant Ser/Thr-specific protein kinases with activity on acidic substrates. In yeast, the products of the redundant YCK1 and YCK2 genes are together essential for cell viability. Mutants deficient for these proteins display defects in cellular morphogenesis, cytokinesis, and endocytosis. Yck1p and Yck2p are peripheral plasma membrane proteins, and we report here that the localization of Yck2p within the membrane is dynamic through the cell cycle. Using a functional green fluorescent protein (GFP) fusion, we have observed that Yck2p is concentrated at sites of polarized growth during bud morphogenesis. At cytokinesis, GFP-Yck2p becomes associated with a ring at the bud neck and then appears as a patch of fluorescence, apparently coincident with the dividing membranes. The bud neck association of Yck2p at cytokinesis does not require an intact septin ring, and septin assembly is altered in a Yck-deficient mutant. The sites of GFP-Yck2p concentration and the defects observed for Yck-deficient cells together suggest that Yck plays distinct roles in morphogenesis and cytokinesis that are effected by differential localization.

MeSH Terms
Casein Kinase I Casein Kinases Cell Cycle/physiology Cell Division Cell Polarity Cloning, Molecular Cytoskeletal Proteins/metabolism Diploidy Green Fluorescent Proteins Haploidy Isoenzymes/biosynthesis,genetics,metabolism Luminescent Proteins/biosynthesis Morphogenesis Mutagenesis Open Reading Frames Protein Kinases/biosynthesis,genetics,metabolism Recombinant Fusion Proteins/biosynthesis,metabolism Saccharomyces cerevisiae/enzymology,genetics,growth & development Saccharomyces cerevisiae Proteins
Chemicals
Cytoskeletal Proteins Isoenzymes Luminescent Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Green Fluorescent Proteins Protein Kinases Casein Kinase I Casein Kinases YCK1 protein, S cerevisiae YCK2 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Robinson L C
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport, Louisiana 71130, USA. [email protected]
Bradley C
Bryan J D
Jerome A
Kweon Y
Panek H R
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.10.4.1077
Email
[email protected]
Published
1999-04-00
Pages
1077-92
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25234
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.10.4.1077 PMC Full Text View journal details
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