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PMID: 11257023 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mdt(A), a new efflux protein conferring multiple antibiotic resistance in Lactococcus lactis and Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 45 ·No. 4 ·2001-04-00 ·Pages 1109-14

Perreten V, Schwarz FV, Teuber M, Levy SB

Abstract

The mdt(A) gene, previously designated mef214, from Lactococcus lactis subsp. lactis plasmid pK214 encodes a protein [Mdt(A) (multiple drug transporter)] with 12 putative transmembrane segments (TMS) that contain typical motifs conserved among the efflux proteins of the major facilitator superfamily. However, it also has two C-motifs (conserved in the fifth TMS of the antiporters) and a putative ATP-binding site. Expression of the cloned mdt(A) gene decreased susceptibility to macrolides, lincosamides, streptogramins, and tetracyclines in L. lactis and Escherichia coli, but not in Enterococcus faecalis or in Staphylococcus aureus. Glucose-dependent efflux of erythromycin and tetracycline was demonstrated in L. lactis and in E. coli.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,physiology Base Sequence Carrier Proteins/genetics,physiology Cloning, Molecular Drug Resistance, Microbial Drug Resistance, Multiple Erythromycin/metabolism Escherichia coli/drug effects,metabolism Escherichia coli Proteins Genes, Bacterial Kinetics Lactococcus lactis/drug effects,genetics,metabolism Membrane Proteins/genetics,physiology Membrane Transport Proteins Molecular Sequence Data Phenotype Tetracycline/metabolism
Chemicals
Bacterial Proteins Carrier Proteins Escherichia coli Proteins Mdt-A protein, E coli Membrane Proteins Membrane Transport Proteins Erythromycin Tetracycline
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Perreten V
Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Schwarz F V
Teuber M
Levy S B
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.45.4.1109-1114.2001
Published
2001-04-00
Pages
1109-14
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC90432
Subset
IM
Grants
NIGMS NIH HHS · GM 51661 · United States
Databases
GENBANK
X92946
External Links
PubMed source DOI: 10.1128/AAC.45.4.1109-1114.2001 PMC Full Text View journal details
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